Application of 460 nm visible light for the elimination of Candida albicans in vitro and in vivo.
Application of 460 nm visible light for the elimination of Candida albicans in vitro and in vivo.
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应用 460 nm 可见光消除体外和体内白色念珠菌
DOI：
10.3892/mmr.2018.9196
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发表时间：
2018-08
影响因子：
3.4
通讯作者：
Dong J
中科院分区：
文献类型：
--
作者：
Wang C;Yang Z;Peng Y;Guo Y;Yao M;Dong J
研究460 nm蓝光对白色念珠菌的体外杀灭作用。小鼠模型中白色念珠菌感染的皮肤伤口。本文研究了BL辐照对C.白念珠菌的体外和体内研究。C.分别用平板法和流式细胞仪检测BL照射后白念珠菌的菌落数和细胞数。为了确定BL是否能根除C.白念珠菌细胞内的生物膜，在体外C.建立白念珠菌生物膜模型，采用激光共聚焦显微镜和Live/Dead染色试剂盒观察BL对白念珠菌生物膜的影响。此外，还建立了感染C.白色念珠菌已建立。伤后0、3、7、10和14天测定伤口愈合率和组织学检查。结果表明，C. BL以剂量依赖性方式根除白色念珠菌，最小通量为60 J/cm 2。BL辐照几乎完全根除C。当光通量为240 J/cm ~ 2时，C.生物膜内的白色念珠菌也被根除，生物膜在240 J/cm 2的BL照射后被破坏。此外，BL还能显著抑制C.体内白色念珠菌感染。BL照射能促进C.白色念珠菌感染的小鼠模型中的皮肤伤口。总之，本研究的结果表明，460 nm BL可以根除嗜酸性和生物膜C。白念珠菌的体外培养，并代表了一种新的治疗策略，用于治疗念珠菌。体内白色念珠菌感染。
The aim of the present study was to investigate the eradicating effects of 460 nm blue light (BL) on Candida albicans in vitro and in C. albicans-infected skin wounds in a mouse model. In the present study, the antifungal effects of irradiation with BL on C. albicans in vitro and in vivo were investigated. C. albicans colonies and cell numbers were investigated using the spread plate method and flow cytometry respectively following treatment with BL irradiation. In order to determine whether BL can eradicate C. albicans cells within biofilms, an in vitro C. albicans biofilm model was established, and the effect of BL was subsequently investigated using a confocal laser scanning microscope and a Live/Dead staining kit. Furthermore, a mouse skin wound infection model infected with C. albicans was established. Wound healing rates and histological examinations were determined 0, 3, 7, 10 and 14 days post-wounding. The results revealed that C. albicans was eradicated by BL in a dose-dependent manner, with a minimum fluence of 60 J/cm2. Irradiation with BL almost completely eradicated C. albicans when the light fluence was 240 J/cm2. C. albicans inside biofilms was also eradicated and biofilms were destroyed following BL irradiation at 240 J/cm2. In addition, BL was revealed to significantly suppress C. albicans infection in vivo. Irradiation with BL promoted the wound healing of C. albicans infected-skin wounds in a mouse model. In conclusion, the results of the present study demonstrated that 460 nm BL may eradicate planktonic and biofilm C. albicans in vitro, and represents a novel therapeutic strategy for the treatment of C. albicans infections in vivo.
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影响因子：
4.9
作者：
Dai, Tianhong;de Arce, Vida J. Bil;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
DOI：
10.3791/56743
发表时间：
2017-12-14
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Gulati M;Ennis CL;Rodriguez DL;Nobile CJ
通讯作者：
Nobile CJ
影响因子：
3.3
作者：
Feuerstein, O;Ginsburg, I;Weiss, EI
通讯作者：
Weiss, EI
影响因子：
7.6
作者：
Chandra, J;Mukherjee, PK;Ghannoum, MA
通讯作者：
Ghannoum, MA
影响因子：
4.9
作者：
Lambrechts, SAG;Aalders, MCG;Van Marle, J
通讯作者：
Van Marle, J