Differential Regulation of Neurite Outgrowth and Growth Cone Morphology by 3D Fibronectin and Fibronectin-Collagen Extracellular Matrices.
Differential Regulation of Neurite Outgrowth and Growth Cone Morphology by 3D Fibronectin and Fibronectin-Collagen Extracellular Matrices.
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DOI：
10.1007/s12035-021-02637-x
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发表时间：
2022-03
影响因子：
5.1
通讯作者：
Schwarzbauer JE
中科院分区：
文献类型：
--
作者：
Sharma A;Schwarzbauer JE
细胞外基质（ECM）在组织结构和功能的发育、稳态和再生中起关键作用。细胞与ECM的相互作用是动态的，并且细胞通过形态学和运动性的变化来响应ECM重塑。在神经再生过程中，ECM促进神经突生长并以靶特异性引导轴突。脱细胞ECM保留了天然ECM的结构、生物化学和生物力学线索，并且具有在组织修复期间替代受损基质以支持细胞活性的潜力。为了确定有助于神经再生的ECM组分，我们分析了两种类型的脱细胞ECM上的神经元-ECM相互作用。一种基质主要由纤连蛋白（FN）原纤维组成，另一种富含FN的ECM也含有大量的I型胶原（COL I）原纤维。使用从上级颈神经节（SCG）外植体分离的原代神经元，我们发现，神经突在两种基质上延伸，24小时后平均神经突长度无显著差异。FN基质上的神经突最显著的特征是许多短的充满肌动蛋白的突起和从神经突轴延伸的较长的分支。在FN-COL基质上检测到非常少的突起和分支。生长锥形态也不同，FN基质上主要是丝状伪足生长锥，而在FN-COL基质上，形成了相等数量的丝状伪足和细长生长锥。我们的工作提供了有关组织修复过程中ECM主要成分的变化如何调节神经元和生长锥形态的新信息，并有助于确定神经元-ECM相互作用对神经发育和再生的贡献。
The extracellular matrix (ECM) plays a critical role in development, homeostasis, and regeneration of tissue structures and functions. Cell interactions with the ECM are dynamic and cells respond to ECM remodeling by changes in morphology and motility. During nerve regeneration, the ECM facilitates neurite outgrowth and guides axons with target specificity. Decellularized ECMs retain structural, biochemical, and biomechanical cues of native ECM and have the potential to replace damaged matrix to support cell activities during tissue repair. To determine the ECM components that contribute to nerve regeneration, we analyzed neuron-ECM interactions on two types of decellularized ECM. One matrix was composed primarily of fibronectin (FN) fibrils, and the other FN-rich ECM also contained significant numbers of type I collagen (COL I) fibrils. Using primary neurons dissociated from superior cervical ganglion (SCG) explants, we found that neurites were extended on both matrices without a significant difference in average neurite length after 24h. The most distinctive features of neurites on the FN matrix were numerous short actin-filled protrusions and longer branches extending from neurite shafts. Very few protrusions and branches were detected on FN-COL matrix. Growth cone morphologies also differed with mostly filopodial growth cones on FN matrix whereas on FN-COL matrix, equivalent numbers of filopodial and slender growth cones were formed. Our work provides new information about how changes in major components of the ECM during tissue repair modulate neuron and growth cone morphologies and helps to define the contributions of neuron-ECM interactions to nerve development and regeneration.
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DOI：
10.1016/j.mcn.2017.03.001
发表时间：
2017-10
期刊：
Molecular and cellular neurosciences
影响因子：
--
作者：
Omotade OF;Pollitt SL;Zheng JQ
通讯作者：
Zheng JQ
影响因子：
--
作者：
Muncie JM;Weaver VM
通讯作者：
Weaver VM
影响因子：
6.9
作者：
Reitman, Irene;Huang, Mia L.;Schwarzbauer, Jean E.
通讯作者：
Schwarzbauer, Jean E.
影响因子：
4
作者：
Mao, Y;Schwarzbauer, JE
通讯作者：
Schwarzbauer, JE
影响因子：
3
作者：
Gardiner, Natalie J.
通讯作者：
Gardiner, Natalie J.