TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T.
TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T.
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DOI：
10.1038/s41467-020-18782-1
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发表时间：
2020-10-12
影响因子：
16.6
通讯作者：
Wagner D
中科院分区：
文献类型：
--
作者：
Zhu Y;Klasfeld S;Jeong CW;Jin R;Goto K;Yamaguchi N;Wagner D
植物监测季节性的线索，以优化生殖成功的调整开始繁殖和花序结构。烟草花1（TFL 1）和FLOWERLOCUS T（FT）及其直系同源物拮抗性调节这些生活史性状，但其作用机制，拮抗作用和靶标仍然知之甚少。在这里，我们发现TFL 1被bZIP转录因子FD招募到数千个位点。我们确定了花命运的主要调节因子，LEAFY（LFY）作为TFL 1和FT双重相反调节下的靶点，并揭示了FT通过LFY上调促进花命运的关键作用。我们提供的证据表明，FT和TFL 1之间的拮抗作用依赖于在共享的靶位点上对染色质结合FD的竞争。直接TFL 1-FD调节的靶基因将该复合物鉴定为抑制生殖发育和内源性信号传导途径的主调节因子的枢纽。我们的数据提供了TFL 1-FD如何塑造花序结构的机理见解，这是一个对繁殖成功，植物结构和产量很重要的性状。开花调节因子TFL 1和FT在开花植物中以拮抗方式同步生殖转变。Zhu等人在本文中表明，TFL 1被bZIP转录因子FD募集到数千个靶基因座，并揭示FT与TFL 1竞争包括LFY在内的共享靶点处的染色质结合FD，FT上调LFY促进花的命运。
Plants monitor seasonal cues to optimize reproductive success by tuning onset of reproduction and inflorescence architecture. TERMINAL FLOWER 1 (TFL1) and FLOWERING LOCUS T (FT) and their orthologs antagonistically regulate these life history traits, yet their mechanism of action, antagonism and targets remain poorly understood. Here, we show that TFL1 is recruited to thousands of loci by the bZIP transcription factor FD. We identify the master regulator of floral fate, LEAFY (LFY) as a target under dual opposite regulation by TFL1 and FT and uncover a pivotal role of FT in promoting flower fate via LFY upregulation. We provide evidence that the antagonism between FT and TFL1 relies on competition for chromatin-bound FD at shared target loci. Direct TFL1-FD regulated target genes identify this complex as a hub for repressing both master regulators of reproductive development and endogenous signalling pathways. Our data provide mechanistic insight into how TFL1-FD sculpt inflorescence architecture, a trait important for reproductive success, plant architecture and yield. The flowering regulators TFL1 and FT act antagonistically to synchronize reproductive transition in flowering plants. Here Zhu et al. show that TFL1 is recruited by the bZIP transcription factor FD to thousands of target loci and reveal that FT competes with TFL1 for chromatin bound-FD at shared targets including LFY, upregulation of which by FT promotes floral fate.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
DOI：
10.1016/j.cub.2010.01.059
发表时间：
2010-04-13
期刊：
Current biology : CB
影响因子：
--
作者：
Blackman BK;Strasburg JL;Raduski AR;Michaels SD;Rieseberg LH
通讯作者：
Rieseberg LH
影响因子：
4.9
作者：
Chen L;Wang F;Wang X;Liu YG
通讯作者：
Liu YG
影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF