WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.
WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.
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DOI：
10.1016/j.stemcr.2013.03.003
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发表时间：
2013
影响因子：
5.9
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
--
作者：
Jiang, Wei;Zhang, Donghui;Bursac, Nenad;Zhang, Yi
人多能干细胞（pluripotent stem cells，PSC）体外诱导分化为功能细胞是一种很有前途的药物筛选和细胞治疗方法。然而，观察到的不同人胚胎干细胞（hESC）/诱导的PSC（iPSC）系的分化潜能的大的且不可避免的变化使得难以选择用于分化特定细胞谱系的适当细胞系。在这里，我们报告了WNT 3作为能够预测hESC定形内胚层（DE）分化潜力的生物标志物的鉴定。我们发现，WNT 3在hESC中的mRNA水平与其DE分化效率相关。此外，通过WNT 3敲低或过表达的hESC的操作可以WNT 3水平依赖性方式分别抑制或促进DE分化。最后，基于其WNT 3表达水平的几个hESC系的分析允许准确预测其DE分化潜力。总的来说，我们的研究支持WNT 3可以作为预测hESC DE分化潜力的生物标志物的观点。hESC中的WNT 3水平与DE分化效率相关hESC中的WNT 3水平不影响多能基因表达hESC中WNT 3水平的操纵可以改变其DE分化潜能。
Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of different human embryonic stem cell (hESC)/induced PSC (iPSC) lines makes the selection of an appropriate cell line for the differentiation of a particular cell lineage difficult. Here, we report identification of WNT3 as a biomarker capable of predicting definitive endoderm (DE) differentiation potential of hESCs. We show that the mRNA level of WNT3 in hESCs correlates with their DE differentiation efficiency. In addition, manipulations of hESCs through WNT3 knockdown or overexpression can respectively inhibit or promote DE differentiation in a WNT3 level-dependent manner. Finally, analysis of several hESC lines based on their WNT3 expression levels allowed accurate prediction of their DE differentiation potential. Collectively, our study supports the notion that WNT3 can serve as a biomarker for predicting DE differentiation potential of hESCs. ► WNT3 level in hESCs correlates with DE differentiation efficiency ► WNT3 level in hESCs does not affect pluripotent gene expression ► Manipulation of WNT3 level in hESCs can change their DE differentiation potential.
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影响因子：
44.1
作者：
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