Wild-type oestrogen receptor beta (ERbeta1) mRNA and protein expression in Tamoxifen-treated post-menopausal breast cancers.
Wild-type oestrogen receptor beta (ERbeta1) mRNA and protein expression in Tamoxifen-treated post-menopausal breast cancers.
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DOI：
10.1038/sj.bjc.6602183
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发表时间：
2004-11-01
影响因子：
8.8
通讯作者：
Foster, CS
中科院分区：
文献类型：
--
作者：
O'Neill, PA;Davies, MPA;Shaaban, AM;Innes, H;Torevell, A;Sibson, DR;Foster, CS
本研究验证了以下假设，即比较人乳腺癌ERα和ERβ1的蛋白和mRNA表达提供了与人乳腺癌的临床和病理特征相关的新信息。对167例仅行内分泌治疗的绝经后妇女浸润性癌进行ERα和ERβ1检测。该队列包括143例手术后仅接受他莫昔芬辅助治疗的病例。采用免疫组化和逆转录RT-PCR方法检测ERα和ERβ1的表达，并与临床分期进行比较。ERα蛋白与RT-PCR检测的相应RNA之间存在显著相关性（χ 2，P<0.001）。ERβ1蛋白和mRNA表达不一致。虽然ERα和ERβ mRNA之间存在相关性（卡方检验，P<0.001），ERα蛋白和ERβ1 mRNA之间存在相关性（卡方检验，P<0.027），但免疫组化检测的ERα和ERβ1蛋白之间没有相关性。ERβ1与预后无关。然而，在缺乏ERα的情况下，ERβ1蛋白表达与细胞增殖的升高相关。ERβ1蛋白阳性病例的结局有恶化的趋势，无论是在整个组内还是在ERα阳性他莫昔芬治疗的病例中。本研究证实了ERα表达是乳腺癌进展的重要决定因素的假设，并进一步证明ERβ1可能在乳腺癌对内分泌治疗的反应中起作用。
This study has tested the hypothesis that comparison of protein and mRNA expression for ERα and ERβ1 by human breast cancers provides novel information relating to the clinical and pathological characteristics of human breast cancers. Expression of ERα and ERβ1 was identified in 167 invasive cancers from postmenopausal women treated only with endocrine therapy. The cohort included 143 cases receiving only adjuvant Tamoxifen following surgery. ERα and ERβ1 expression was analysed by immunohistochemistry and reverse transcription RT–PCR and compared with clinical progression of individual cancers. ERα protein was closely associated with the corresponding RNA detected by RT–PCR (Chi-square, P<0.001). In contrast, ERβ1 protein and mRNA were inconsistent. Although an association was identified between ERα and ERβ mRNAs (Chi-square, P<0.001) and between ERα protein and ERβ1 mRNA (Chi-square, P<0.027), no association was identified for the ERα and ERβ1 proteins detected by immunohistochemistry. ERβ1 was not associated with outcome. However, in the absence of ERα, ERβ1 protein expression was associated with elevated cell proliferation. There was a trend for the ERβ1 protein-positive cases to have a worse outcome, both within the group as a whole as well as within the ERα-positive Tamoxifen-treated cases. This study has confirmed the hypothesis that expression of ERα is an important determinant of breast cancer progression, and has further demonstrated that ERβ1 may play a role in the response of breast cancers to endocrine therapy.
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影响因子：
4.1
作者：
Choi, I;Ko, C;Katzenellenbogen, BS
通讯作者：
Katzenellenbogen, BS
影响因子：
7.3
作者：
Shaw, JA;Udokang, K;Walker, RA
通讯作者：
Walker, RA
DOI：
10.1006/bbrc.1998.8738
发表时间：
1998-06-09
影响因子：
3.1
作者：
Moore, JT;McKee, DD;Willson, TM
通讯作者：
Willson, TM
影响因子：
56.9
作者：
Paech, K;Webb, P;Scanlan, TS
通讯作者：
Scanlan, TS
影响因子：
7.3
作者：
Skliris, GP;Parkes, AT;Speirs, V
通讯作者：
Speirs, V