Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa L.)
Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa L.)
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水稻 (Oryza sativa L.) 病斑模拟基因斑点叶 5 (spl5) 的精细遗传图谱和物理定界
DOI：
10.1007/s11032-009-9299-2
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发表时间：
2009-06
影响因子：
3.1
通讯作者：
Zhai,W.
中科院分区：
文献类型：
--
作者：
Pan,J.;Jin,Y.;Gu,Z.;Cheng,J.;Chen,X.;Jiang,G.;Ma,B.;Qian,Q.;Zhai,W.
斑点叶5（spl 5）是一种模拟病斑突变体，首次在水稻（Oryza sativa L.）日本1978年的Norin 8。该突变体在没有任何病原体的情况下表现出自发的疾病样病变，并且对稻瘟病和白叶枯病具有抗性;然而，目的基因尚未被分离。在本研究中，我们采用了基于图位的克隆策略，精细定位spl 5基因。在用100个由spl 5突变体和籼稻cv. 93-11中，利用6个SSR标记将spl 5基因定位在第7染色体上的3.3cM区域。在一个高分辨率的遗传作图中，两个F2群体（spl 5/spl 5）的3，149个个体来自两个spl 5突变体和两个籼型cvs的杂交。93-11和浙辐802等5个新标记以及6个序列标记位点（STS）标记。最后，将spl 5基因定位到两个标记SSR 7和RM 7121之间的0.048 cM区域。通过Pairwise BLAST分析构建了一张涵盖这些标记位点和spl 5基因的BAC/PAC重叠群图谱。我们的生物信息学分析表明，spl 5基因位于两个标记SSR 7和RM 7121之间的80 kb区域，具有较高的平均物理遗传距离比（1.67 Mb/cM）和18个候选基因。
Spotted leaf 5 (spl5), a lesion mimic mutant, was first identified in rice (Oryza sativa L.) japonica cv. Norin8 in 1978. This mutant exhibits spontaneous disease-like lesions in the absence of any pathogens and resistance to rice blast and bacterial blight; however, the target gene has not yet been isolated. In the present study, we employed a map-based cloning strategy to finely map the spl5 gene. In an initial mapping with 100 F2 individuals (spl5/spl5) derived from a cross between the spl5 mutant and indica cv. 93-11, the spl5 gene was located in a 3.3-cM region on chromosome 7 using six simple sequence repeat (SSR) markers. In a high-resolution genetic mapping, two F2 populations with 3,149 individuals (spl5/spl5) were derived from two crosses between spl5 mutant and two indica cvs. 93-11 and Zhefu802 and six sequence-tagged site (STS) markers were newly developed. Finally, the spl5 gene was mapped to a region of 0.048 cM between two markers SSR7 and RM7121. One BAC/PAC contig map covering these markers’ loci and the spl5 gene was constructed through Pairwise BLAST analysis. Our bioinformatics analysis shows that the spl5 gene is located in the 80-kb region between two markers SSR7 and RM7121 with a high average ratio of physical to genetic distance (1.67 Mb/cM) and eighteen candidate genes.
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影响因子：
5.2
作者：
Mizobuchi, R;Hirabayashi, H;Okamoto, M
通讯作者：
Okamoto, M
影响因子：
3.1
作者：
Changjian Wu;A. Bordeos;M. Madamba;M. Baraoidan;M. Ramos;Guo‐Liang Wang;J. Leach;H. Leung
通讯作者：
Changjian Wu;A. Bordeos;M. Madamba;M. Baraoidan;M. Ramos;Guo‐Liang Wang;J. Leach;H. Leung
影响因子：
--
作者：
H. Vos;Alex W. Faber;Maaike D de Gier;J. C. Vos;H. A. Raué
通讯作者：
H. Vos;Alex W. Faber;Maaike D de Gier;J. C. Vos;H. A. Raué
DOI：
10.1007/s11427-008-0044-6
发表时间：
2008-03
期刊：
Science in China Series C: Life Sciences
影响因子：
--
作者：
Chunmei Wang;Huiwen Wang;Jin‐Song Zhang;S. Chen
通讯作者：
Chunmei Wang;Huiwen Wang;Jin‐Song Zhang;S. Chen
影响因子：
2.7
作者：
HOISINGTON, DA;NEUFFER, MG;WALBOT, V
通讯作者：
WALBOT, V