Development and physiological effects of an artificial diet for Wolbachia-infected Aedes aegypti.
Development and physiological effects of an artificial diet for Wolbachia-infected Aedes aegypti.
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DOI：
10.1038/s41598-017-16045-6
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发表时间：
2017-11-16
影响因子：
4.6
通讯作者：
Moreira LA
中科院分区：
文献类型：
--
作者：
Dutra HLC;Rodrigues SL;Mansur SB;de Oliveira SP;Caragata EP;Moreira LA
内共生细菌沃尔巴克氏体在埃及伊蚊种群中迅速传播，并强烈抑制包括登革热和寨卡病毒在内的主要人类病原体的感染。旨在限制这些病毒传播的蚊子控制计划正在多个国家进行，但缺乏专门针对沃尔巴克氏体感染蚊子的大规模饲养基础设施。一个例子是缺乏血粉替代品，这是受感染蚊子体内沃尔巴克氏体特异性生理变化的原因，这种变化允许细菌传播，并阻止病毒感染。为此，我们开发了一种血粉替代品，专门用于感染wMel Wolbachia菌株的蚊子。这种饮食，ADM，含有乳蛋白和婴儿配方，溶解在牛红细胞和伊蚊生理盐水的混合物中，ATP作为诱食剂。用ADM喂养导致高水平的活卵产量，但也不影响关键的沃尔巴克氏体参数，包括细菌密度、细胞质不相容性或对寨卡病毒感染的抵抗力。ADM是一种有效的人血替代品，可用于大规模饲养wMel感染的A.并且在将来可以容易地被优化以提高性能。
The endosymbiotic bacterium Wolbachia spreads rapidly through populations of Aedes aegypti mosquitoes, and strongly inhibits infection with key human pathogens including the dengue and Zika viruses. Mosquito control programs aimed at limiting transmission of these viruses are ongoing in multiple countries, yet there is a dearth of mass rearing infrastructure specific to Wolbachia-infected mosquitoes. One example is the lack of a blood meal substitute, which accounts for the Wolbachia-specific physiological changes in infected mosquitoes, that allows the bacterium to spread, and block viral infections. To that end, we have developed a blood meal substitute specifically for mosquitoes infected with the wMel Wolbachia strain. This diet, ADM, contains milk protein, and infant formula, dissolved in a mixture of bovine red blood cells and Aedes physiological saline, with ATP as a phagostimulant. Feeding with ADM leads to high levels of viable egg production, but also does not affect key Wolbachia parameters including, bacterial density, cytoplasmic incompatibility, or resistance to infection with Zika virus. ADM represents an effective substitute for human blood, which could potentially be used for the mass rearing of wMel-infected A. aegypti, and could easily be optimized in the future to improve performance.
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影响因子：
3.8
作者：
Caragata EP;Rezende FO;Simões TC;Moreira LA
通讯作者：
Moreira LA
DOI：
10.1073/pnas.1116932108
发表时间：
2012-01-03
影响因子：
11.1
作者：
Pan, Xiaoling;Zhou, Guoli;Xi, Zhiyong
通讯作者：
Xi, Zhiyong
影响因子：
3.8
作者：
Dutra HL;Dos Santos LM;Caragata EP;Silva JB;Villela DA;Maciel-de-Freitas R;Moreira LA
通讯作者：
Moreira LA
影响因子：
6.7
作者：
Caragata EP;Rancès E;Hedges LM;Gofton AW;Johnson KN;O'Neill SL;McGraw EA
通讯作者：
McGraw EA
影响因子：
6.7
作者：
Serbus LR;White PM;Silva JP;Rabe A;Teixeira L;Albertson R;Sullivan W
通讯作者：
Sullivan W