From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
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DOI：
10.1371/journal.pntd.0003689
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发表时间：
2015-04
影响因子：
3.8
通讯作者：
Moreira LA
中科院分区：
文献类型：
--
作者：
Dutra HL;Dos Santos LM;Caragata EP;Silva JB;Villela DA;Maciel-de-Freitas R;Moreira LA
共生细菌沃尔巴克氏体目前正在几个国家作为生物控制剂进行试验，以减少登革热的传播。沃尔巴克氏体可以入侵和传播，感染野生蚊子种群中的所有个体，但需要高的母体传播率，强的细胞质不相容性和宿主中的低适应性成本。此外，广泛的差异，在气候，现场释放协议，城市化水平和人口密度之间的网站，这种细菌已经部署了有限的比较和分析沃尔巴克氏体的入侵潜力。我们研究了wMel Wolbachia菌株在具有巴西遗传背景的实验室埃及伊蚊中的关键表型效应，以表征其入侵潜力。我们表明，wMel株引起强烈的细胞质不亲和性，高的孕产妇传播率，并没有明显的有害影响，对主机的繁殖力或生育力。接下来，为了了解不同城市景观对蚊子生存可能性的影响，我们在里约热内卢的两个地区使用未感染沃尔巴克氏体的巴西蚊子进行了标记释放再捕获实验，其中沃尔巴克氏体将在未来部署。我们的特点，蚊子种群的社会人口条件在这些网站，并在其他三个未来的释放领域。然后，我们使用实验室和现场数据构建数学模型，并使用这些来描述城市环境条件对蚊子释放后Wolbachia感染频率可能达到100%的可能性的影响。我们预测在所有五个现场的成功入侵，但发生这种情况的条件差异很大，网站之间，并强烈影响当地蚊子种群的大小。通过对实验室、现场和数学数据的分析，我们表明，沃尔巴克氏体的wMel菌株具有在里约热内卢不同的城市社会人口环境中有效传播所需的特征，包括消除登革热计划释放蚊子的地方。 沃尔巴克氏体是母体遗传的细菌内共生体，其在蚊子中诱导细胞质不相容性，并利用这种不育模式促进其传播到幼稚的未感染宿主群体中。这些细菌最近被引入到埃及伊蚊中，埃及伊蚊是一种天然无沃尔巴克氏体的物种，也是登革热病毒的主要载体。沃尔巴克氏体目前被用作控制登革热传播的生物工具，包括巴西在内的许多国家。我们进行了一系列的实验室和现场测定，然后建立数学模型，以了解在巴西Ae的wMel沃尔巴克氏体菌株的入侵能力。埃及蚊子种群。我们表明，wMel感染没有有害的健身影响其新的巴西主机，理论上能够成功地入侵蚊子种群在五个不同的城市景观，我们研究。我们发现沃尔巴克氏体在当地蚊子数量较少的地方更容易传播。当地人口的规模并没有明显影响传播的可能性。这些发现提供了对不同城市景观的性质将如何影响沃尔巴克氏体有效传播的可能性的深入了解，这将极大地有助于未来在巴西释放沃尔巴克氏体感染的蚊子，作为消除登革热计划的一部分。
The symbiotic bacterium Wolbachia is currently being trialled as a biocontrol agent in several countries to reduce dengue transmission. Wolbachia can invade and spread to infect all individuals within wild mosquito populations, but requires a high rate of maternal transmission, strong cytoplasmic incompatibility and low fitness costs in the host in order to do so. Additionally, extensive differences in climate, field-release protocols, urbanization level and human density amongst the sites where this bacterium has been deployed have limited comparison and analysis of Wolbachia’s invasive potential. We examined key phenotypic effects of the wMel Wolbachia strain in laboratory Aedes aegypti mosquitoes with a Brazilian genetic background to characterize its invasive potential. We show that the wMel strain causes strong cytoplasmic incompatibility, a high rate of maternal transmission and has no evident detrimental effect on host fecundity or fertility. Next, to understand the effects of different urban landscapes on the likelihood of mosquito survival, we performed mark-release-recapture experiments using Wolbachia-uninfected Brazilian mosquitoes in two areas of Rio de Janeiro where Wolbachia will be deployed in the future. We characterized the mosquito populations in relation to the socio-demographic conditions at these sites, and at three other future release areas. We then constructed mathematical models using both the laboratory and field data, and used these to describe the influence of urban environmental conditions on the likelihood that the Wolbachia infection frequency could reach 100% following mosquito release. We predict successful invasion at all five field sites, however the conditions by which this occurs vary greatly between sites, and are strongly influenced by the size of the local mosquito population. Through analysis of laboratory, field and mathematical data, we show that the wMel strain of Wolbachia possesses the characteristics required to spread effectively in different urban socio-demographic environments in Rio de Janeiro, including those where mosquito releases from the Eliminate Dengue Program will take place. Wolbachia are maternally inherited bacterial endosymbionts that induce cytoplasmic incompatibility in mosquitoes, and use this pattern of sterility to facilitate their spread into naïve, uninfected host populations. These bacteria were recently introduced into Aedes aegypti, a species naturally free of Wolbachia, and the primary vector of dengue virus. Wolbachia are currently being used as a biological tool to control dengue transmission in many countries, including Brazil. We performed a series of laboratory and field assays and then created mathematical models in order to understand the invasive ability of the wMel Wolbachia strain in Brazilian Ae. aegypti mosquito populations. We show that wMel infection had no detrimental fitness effects on its new Brazilian host, and would theoretically be able to successfully invade the mosquito populations in the five distinct urban landscapes that we studied. We found that Wolbachia spread more easily where the local mosquito population was smaller. The size of the local human population did not overtly influence the likelihood of spread. These findings provide insight into how the nature of different urban landscapes will affect the likelihood of Wolbachia spreading effectively, which will greatly assist future releases of Wolbachia-infected mosquitoes in Brazil as part of the Eliminate Dengue Program.
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影响因子：
1.9
作者：
Hoffmann, A. A.;Goundar, A. A.;Ritchie, S. A.
通讯作者：
Ritchie, S. A.
影响因子：
3.8
作者：
Hoffmann AA;Iturbe-Ormaetxe I;Callahan AG;Phillips BL;Billington K;Axford JK;Montgomery B;Turley AP;O'Neill SL
通讯作者：
O'Neill SL
DOI：
10.1073/pnas.94.20.10792
发表时间：
1997-09-30
影响因子：
11.1
作者：
Min, KT;Benzer, S
通讯作者：
Benzer, S
影响因子：
3.8
作者：
Keeling, MJ;Jiggins, FM;Read, JM
通讯作者：
Read, JM
影响因子：
56.9
作者：
Keeling, MJ;Woolhouse, MEJ;Grenfell, BT
通讯作者：
Grenfell, BT