Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti.
Diet-Induced Nutritional Stress and Pathogen Interference in Wolbachia-Infected Aedes aegypti.
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DOI：
10.1371/journal.pntd.0005158
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发表时间：
2016-11
影响因子：
3.8
通讯作者：
Moreira LA
中科院分区：
文献类型：
--
作者：
Caragata EP;Rezende FO;Simões TC;Moreira LA
病原体干扰表型在很大程度上限制了感染登革病毒(DENV)和其他病原体的埃及伊蚊的感染，是以沃尔巴克氏菌为基础的蚊子控制的重要组成部分。关键的是，这种表型的原因机制很复杂，而且人们对此知之甚少，最近的证据表明，原因可能是物种特有的。为了更好地了解这一重要的表型，我们研究了饮食诱导的营养应激在沃尔巴克氏菌感染的Ae中对DENV和禽类疟疾寄生虫的干扰的作用。埃及伊蚊，以及与表型相关的生理过程。被沃尔巴克氏杆菌感染的蚊子被喂以四种不同浓度的蔗糖中的一种，然后用鸡疫霉或DENV攻击。改变饲料对鸡疫霉的干扰作用显着减弱，而对DENV的干扰作用不明显。免疫基因表达和过氧化氢水平以前被认为与病原体干扰有关。使用RT-qPCR和Amplex Red过氧化氢/过氧化物酶分析试剂盒对每种饮食中蚊子的这些特征进行了检测，结果发现，饮食的变化对免疫表达没有显著影响，但低碳水化合物水平会导致感染Wolbachia的蚊子失去ROS诱导。我们的数据表明，宿主营养可能不会影响对沃尔巴克氏菌感染蚊子的DENV干扰，但疟原虫的干扰可能与营养和氧化应激有关。这种对营养变化的病原体特异性反应突出了蚊子中沃尔巴克氏菌和病原体之间相互作用的复杂性质。蚊子传播的疾病严重影响着世界各地的人类健康。一种新的控制方式是用自然产生的沃尔巴克氏菌感染医学上重要的蚊子物种，沃尔巴克氏菌通过一种名为病原体干扰的过程限制登革热和疟疾的传播。干扰表型仍然知之甚少，可能涉及蚊子的多种生理变化。为了更好地了解可能导致可变干扰的因素，我们研究了营养应激在登革热媒介埃及伊蚊病原体干扰中的作用。我们证明了对引起疟疾的鸡疟原虫的干扰取决于蚊子的营养状况，而对登革热的干扰不依赖于蚊子的营养状态，这意味着病原体对不同的病原体的干预作用是不同的。然后，我们研究了以前与病原体干扰相关的蚊子免疫过程，并证明蚊子营养并不影响关键蚊子免疫基因的表达，但确实影响了活性氧的水平。我们的结果强调了表型的复杂性，重要的是表明成人营养可能不是干扰DENV的关键决定因素。
The pathogen interference phenotype greatly restricts infection with dengue virus (DENV) and other pathogens in Wolbachia-infected Aedes aegypti, and is a vital component of Wolbachia-based mosquito control. Critically, the phenotype’s causal mechanism is complex and poorly understood, with recent evidence suggesting that the cause may be species specific. To better understand this important phenotype, we investigated the role of diet-induced nutritional stress on interference against DENV and the avian malarial parasite Plasmodium gallinaceum in Wolbachia-infected Ae. aegypti, and on physiological processes linked to the phenotype. Wolbachia-infected mosquitoes were fed one of four different concentrations of sucrose, and then challenged with either P. gallinaceum or DENV. Interference against P. gallinaceum was significantly weakened by the change in diet however there was no effect on DENV interference. Immune gene expression and H2O2 levels have previously been linked to pathogen interference. These traits were assayed for mosquitoes on each diet using RT-qPCR and the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit, and it was observed that the change in diet did not significantly affect immune expression, but low carbohydrate levels led to a loss of ROS induction in Wolbachia-infected mosquitoes. Our data suggest that host nutrition may not influence DENV interference for Wolbachia-infected mosquitoes, but Plasmodium interference may be linked to both nutrition and oxidative stress. This pathogen-specific response to nutritional change highlights the complex nature of interactions between Wolbachia and pathogens in mosquitoes. Mosquito-transmitted disease severely impacts human health around the world. One novel form of control involves infecting medically important mosquito species with the naturally occurring bacterium Wolbachia, which restricts dengue and malaria transmission through a process called pathogen interference. The interference phenotype is still poorly understood, and potentially involves multiple physiological changes to the mosquito. We examined the role of nutritional stress on pathogen interference in the dengue vector Aedes aegypti, in order to better understand factors that might lead to variable interference. We demonstrated that interference against malaria-causing Plasmodium gallinaceum was dependent on mosquito nutritional status, however interference against dengue was not, implying that pathogen interference operates differently for different pathogens. We then examined mosquito immune processes that had been previously correlated with pathogen interference, and demonstrated that mosquito nutrition did not affect the expression of key mosquito immune genes, but did affect levels of reactive oxygen species. Our results highlight the complexity of the phenotype, and importantly suggest that adult nutrition may not be a key determinant of interference against DENV.
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影响因子：
3.7
作者：
Hume JC;Hamilton H 3rd;Lee KL;Lehmann T
通讯作者：
Lehmann T
影响因子：
3.7
作者：
Brown, Anthony E.;Baumbach, Janina;Ligoxygakis, Petros
通讯作者：
Ligoxygakis, Petros
影响因子：
4.4
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
通讯作者：
Collins FH
DOI：
10.1073/pnas.1412984112
发表时间：
2015-01-13
影响因子：
11.1
作者：
Carissimo, Guillaume;Pondeville, Emilie;Vernick, Kenneth D.
通讯作者：
Vernick, Kenneth D.
影响因子：
3.2
作者：
Amuzu, Hilaria E.;Simmons, Cameron P.;McGraw, Elizabeth A.
通讯作者：
McGraw, Elizabeth A.