SARS-CoV-2 Distribution in Residential Housing Suggests Contact Deposition and Correlates with Rothia sp.
SARS-CoV-2 Distribution in Residential Housing Suggests Contact Deposition and Correlates with Rothia sp.
复制标题
DOI：
10.1128/msystems.01411-21
复制
发表时间：
2022-06-28
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
作者：
在表面监测严重急性呼吸综合征冠状病毒2(SARS-CoV-2)正在成为识别过去接触过病毒RNA的个人的重要工具。我们过去的工作表明，来自表面的SARS-CoV-2反转录-定量聚合酶链式反应(RT-qPCR)信号可以识别感染者何时接触表面，以及他们何时出现在医院房间或学校。然而，表面采样作为检测SARS-CoV-2阳性个体存在的一种方法的敏感性和特异性以及在哪里采样的指导尚未确定。为了解决这些问题并检验我们过去的观察是否将SARS-CoV-2的丰度与Rothia sp.在同样是住宅环境的医院，我们对三个隔离单元进行了详细的空间抽样，通过RT-qPCR评估每个样本的SARS-CoV-2丰度，将结果与16S rRNA基因扩增序列(以评估每个地点的细菌群落)以及同期临床测试的CQ值联系起来。我们的结果显示，在这种情况下，SARS-CoV-2病毒载量最高的是接触到的表面，如电灯开关和水龙头，但在许多未接触的表面(如地板)存在可检测到的信号，这些表面可能与环境更相关，如学校强制戴口罩。在过去的研究中，细菌群落预测哪些样本是SARS-CoV-2阳性，与Rothia sp.表现出积极的联系。表面采样对检测导致2019年冠状病毒病的SARS-CoV-2病毒的重要性(新冠肺炎)正越来越多地被用于定位感染者。我们从感染者居住的三个住宅单元收集了381个样本，测试了哪些室内表面病毒载量较高或较低，并根据SARS-CoV-2可能直接(例如，触摸电灯开关)还是间接(例如，通过飞沫或气溶胶沉淀)传播的情况来解释结果。我们发现受试者直接接触表面的负载最高，尽管在间接接触的表面上检测到足够的病毒，使这些位置对采样有用(例如，在学校，学生不接触电灯开关，还戴着口罩，这样他们就没有机会触摸他们的脸，然后是物体)。我们还记录了样本中存在的细菌与SARS-CoV-2病毒之间的联系，这与早期的研究一致。
Monitoring severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on surfaces is emerging as an important tool for identifying past exposure to individuals shedding viral RNA. Our past work demonstrated that SARS-CoV-2 reverse transcription-quantitative PCR (RT-qPCR) signals from surfaces can identify when infected individuals have touched surfaces and when they have been present in hospital rooms or schools. However, the sensitivity and specificity of surface sampling as a method for detecting the presence of a SARS-CoV-2 positive individual, as well as guidance about where to sample, has not been established. To address these questions and to test whether our past observations linking SARS-CoV-2 abundance to Rothia sp. in hospitals also hold in a residential setting, we performed a detailed spatial sampling of three isolation housing units, assessing each sample for SARS-CoV-2 abundance by RT-qPCR, linking the results to 16S rRNA gene amplicon sequences (to assess the bacterial community at each location), and to the Cq value of the contemporaneous clinical test. Our results showed that the highest SARS-CoV-2 load in this setting is on touched surfaces, such as light switches and faucets, but a detectable signal was present in many untouched surfaces (e.g., floors) that may be more relevant in settings, such as schools where mask-wearing is enforced. As in past studies, the bacterial community predicts which samples are positive for SARS-CoV-2, with Rothia sp. showing a positive association. IMPORTANCE Surface sampling for detecting SARS-CoV-2, the virus that causes coronavirus disease 2019 (COVID-19), is increasingly being used to locate infected individuals. We tested which indoor surfaces had high versus low viral loads by collecting 381 samples from three residential units where infected individuals resided, and interpreted the results in terms of whether SARS-CoV-2 was likely transmitted directly (e.g., touching a light switch) or indirectly (e.g., by droplets or aerosols settling). We found the highest loads where the subject touched the surface directly, although enough virus was detected on indirectly contacted surfaces to make such locations useful for sampling (e.g., in schools, where students did not touch the light switches and also wore masks such that they had no opportunity to touch their face and then the object). We also documented links between the bacteria present in a sample and the SARS-CoV-2 virus, consistent with earlier studies.
登录
查看更多内容
影响因子：
14.2
作者：
Ben-Shmuel, Amir;Brosh-Nissimov, Tal;Weiss, Shay
通讯作者：
Weiss, Shay
室内灰尘作为矩阵，用于监视COVID-19。
影响因子：
6.4
作者：
Renninger N;Nastasi N;Bope A;Cochran SJ;Haines SR;Balasubrahmaniam N;Stuart K;Bivins A;Bibby K;Hull NM;Dannemiller KC
通讯作者：
Dannemiller KC
影响因子：
6.4
作者：
Salido RA;Cantú VJ;Clark AE;Leibel SL;Foroughishafiei A;Saha A;Hakim A;Nouri A;Lastrella AL;Castro-Martínez A;Plascencia A;Kapadia BK;Xia B;Ruiz CA;Marotz CA;Maunder D;Lawrence ES;Smoot EW;Eisner E;Crescini ES;Kohn L;Franco Vargas L;Chacón M;Betty M;Machnicki M;Wu MY;Baer NA;Belda-Ferre P;De Hoff P;Seaver P;Ostrander RT;Tsai R;Sathe S;Aigner S;Morgan SC;Ngo TT;Barber T;Cheung W;Carlin AF;Yeo GW;Laurent LC;Fielding-Miller R;Knight R
通讯作者：
Knight R
影响因子：
11.8
作者：
Zhou, Jie;Otter, Jonathan A.;Barclay, Wendy S.
通讯作者：
Barclay, Wendy S.
影响因子：
6.4
作者：
Amir A;McDonald D;Navas-Molina JA;Kopylova E;Morton JT;Zech Xu Z;Kightley EP;Thompson LR;Hyde ER;Gonzalez A;Knight R
通讯作者：
Knight R