A pooled testing system to rapidly identify cattle carrying the elite controller BoLA-DRB3*009:02 haplotype against bovine leukemia virus infection.
A pooled testing system to rapidly identify cattle carrying the elite controller BoLA-DRB3*009:02 haplotype against bovine leukemia virus infection.
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DOI：
10.1111/tan.14502
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发表时间：
2022-01
期刊：
影响因子：
8
通讯作者：
Sekiguchi, Satoshi
中科院分区：
文献类型：
--
作者：
Notsu, Kosuke;El Daous, Hala;Mitoma, Shuya;Norimine, Junzo;Sekiguchi, Satoshi
关键词：
作为对基因具有抵抗力的个体，人类免疫缺陷病毒感染的“精英控制器”(ECs)一直被视为开发进一步医学功能治疗的关键。在畜牧业生产领域，需要鉴定牛白血病病毒(BLV)感染的内皮细胞，以阻断BLV在养殖场的传播链。携带牛白细胞抗原(BOLA)-DRB3*009：02等位基因(DRB3*009：02)的牛极有可能成为BLV ECs。大多数携带该等位基因的牛保持着无法检测到的BLV前病毒载量，即使受到感染也不会排毒。BLV ECs在牛舍中放置在未感染和感染的牛之间时，可以起到传播屏障的作用。为了更容易地在大种群中识别携带DRB3*009：02的牛，我们开发了一种联合检测系统。它采用了高灵敏、特异的实时聚合酶链式反应和TaqMan MGB探针(DRB3*009：02-TaqMan检测)。利用这个系统，我们测定了日本九州岛DRB3*009：02携带牛的百分比。我们的混合检测系统从DNA池中检测到携带DRB3*009：02等位基因的牛，该DNA池包含一头DRB3*009：02阳性牛和29头带有其他等位基因的牛。它的能力足以对携带DRB3*009：02的牛进行牛群级别的筛查。DRB3*009：02-TaqMan方法对DRB3*009：02具有较高的鉴别灵敏度和特异性，适用于个体筛查后鉴定携带DRB3*009：02的牛。经测定，九州岛携带DRB3*009：02牛的比例为10.56%。这种新方法使用方便，检测可靠，加强了DRB3*009：02携带牛的实验室分型。因此，我们的研究结果支持在现场使用BLV ECs。
As genetically resistant individuals, the “elite controllers” (ECs) of human immunodeficiency virus infection have been focused on as the keys to developing further functional treatments in medicine. In the livestock production field, identifying the ECs of bovine leukemia virus (BLV) infection in cattle is desired to stop BLV transmission chains on farms. Cattle carrying the bovine leukocyte antigen (BoLA)‐DRB3*009:02 allele (DRB3*009:02) have a strong possibility of being BLV ECs. Most of cattle carrying this allele maintain undetectable BLV proviral loads and do not shed virus even when infected. BLV ECs can act as transmission barriers when placed between uninfected and infected cattle in a barn. To identify cattle carrying DRB3*009:02 in large populations more easily, we developed a pooled testing system. It employs a highly sensitive, specific real‐time PCR assay and TaqMan MGB probes (DRB3*009:02‐TaqMan assay). Using this system, we determined the percentage of DRB3*009:02‐carrying cattle on Kyushu Island, Japan. Our pooled testing system detected cattle carrying the DRB3*009:02 allele from a DNA pool containing one DRB3*009:02‐positive animal and 29 cattle with other alleles. Its capacity is sufficient for herd‐level screening for DRB3*009:02‐carrying cattle. The DRB3*009:02‐TaqMan assay showed high‐discriminative sensitivity and specificity toward DRB3*009:02, making it suitable for identifying DRB3*009:02‐carrying cattle in post‐screening tests on individuals. We determined that the percentage of DRB3*009:02‐carrying cattle in Kyushu Island was 10.56%. With its ease of use and reliable detection, this new method strengthens the laboratory typing for DRB3*009:02‐carrying cattle. Thus, our findings support the use of BLV ECs in the field.
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影响因子：
2.6
作者：
Kobayashi S;Tsutsui T;Yamamoto T;Hayama Y;Kameyama K;Konishi M;Murakami K
通讯作者：
Murakami K
DOI：
10.3390/v7112929
发表时间：
2015-11-24
期刊：
Viruses
影响因子：
--
作者：
Barez PY;de Brogniez A;Carpentier A;Gazon H;Gillet N;Gutiérrez G;Hamaidia M;Jacques JR;Perike S;Neelature Sriramareddy S;Renotte N;Staumont B;Reichert M;Trono K;Willems L
通讯作者：
Willems L
DOI：
10.1084/jem.20070784
发表时间：
2007-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Almeida JR;Price DA;Papagno L;Arkoub ZA;Sauce D;Bornstein E;Asher TE;Samri A;Schnuriger A;Theodorou I;Costagliola D;Rouzioux C;Agut H;Marcelin AG;Douek D;Autran B;Appay V
通讯作者：
Appay V
影响因子：
3.5
作者：
Juliarena, Marcela A.;Barrios, Clarisa N.;Esteban, Eduardo N.
通讯作者：
Esteban, Eduardo N.
影响因子：
5.2
作者：
Aida Y;Murakami H;Takahashi M;Takeshima SN
通讯作者：
Takeshima SN