Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis.
Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility in Fragaria viridis.
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转录组学和蛋白质组学联合分析揭示了草莓自体花粉管发育中涉及的多种途径以及 FviYABBY1 在自交不亲和性中的潜在作用
DOI：
10.3389/fpls.2022.927001
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
草莓配子体自交不亲和性以S-RNase为基础，S-RNase是抑制花粉管生长的主要因子。然而，参与的途径和直接原因的抑制花粉管生长仍然未知。在这里，交互式RNA测序和蛋白质组分析揭示了F。在自花授粉后0和24 h收获viridis花柱。共鉴定出2,181个差异表达基因和200个差异蛋白质。进一步挖掘了与自交不亲和授粉相关的差异表达基因和差异蛋白质，发现其参与了多种途径。有趣的是，与极性生长相关的转录因子FviYABBY 1的表达模式与同一家族中的其他基因不同。FviYABBY 1在花粉中的表达量极高，其在自花授粉花柱中的表达趋势与S-RNase一致。FviYABBY 1与S-RNase的相互作用方式为非S单倍型。因此，FviYABBY 1影响自交花粉管中极性生长相关基因的表达，并受S-RNase正调控。
Fragaria viridis exhibits S-RNase-based gametophytic self-incompatibility, in which S-RNase is the major factor inhibiting pollen tube growth. However, the pathways involved in and the immediate causes of the inhibition of pollen tube growth remain unknown. Here, interactive RNA sequencing and proteome analysis revealed changes in the transcriptomic and proteomic profiles of F. viridis styles harvested at 0 and 24 h after self-pollination. A total of 2,181 differentially expressed genes and 200 differentially abundant proteins were identified during the pollen development stage of self-pollination. Differentially expressed genes and differentially abundant proteins associated with self-incompatible pollination were further mined, and multiple pathways were found to be involved. Interestingly, the expression pattern of the transcription factor FviYABBY1, which is linked to polar growth, differed from those of other genes within the same family. Specifically, FviYABBY1 expression was extremely high in pollen, and its expression trend in self-pollinated styles was consistent with that of S-RNase. Furthermore, FviYABBY1 interacted with S-RNase in a non-S haplotype way. Therefore, FviYABBY1 affects the expression of polar growth-related genes in self-pollen tubes and is positively regulated by S-RNase.
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影响因子：
3.7
作者：
Aguiar B;Vieira J;Cunha AE;Fonseca NA;Iezzoni A;van Nocker S;Vieira CP
通讯作者：
Vieira CP
植物的衰老和程序性细胞死亡：转谷氨酰胺酶介导的多胺作用。
影响因子：
5.6
作者：
Del Duca S;Serafini-Fracassini D;Cai G
通讯作者：
Cai G
影响因子：
11.4
作者：
del Pozo, O;Pedley, KF;Martin, GB
通讯作者：
Martin, GB
影响因子：
--
作者：
Elleman, CJ;Dickinson, HG
通讯作者：
Dickinson, HG
影响因子：
3.9
作者：
Bartholmes, C.;Hidalgo, O.;Gleissberg, S.
通讯作者：
Gleissberg, S.