Lentiviral vector delivery of human interleukin-7 (hIL-7) to human immune system (HIS) mice expands T lymphocyte populations.
Lentiviral vector delivery of human interleukin-7 (hIL-7) to human immune system (HIS) mice expands T lymphocyte populations.
复制标题
DOI：
10.1371/journal.pone.0012009
复制
发表时间：
2010-08-06
期刊：
影响因子：
3.7
通讯作者：
Baltimore D
中科院分区：
文献类型：
--
作者：
O'Connell RM;Balazs AB;Rao DS;Kivork C;Yang L;Baltimore D
Genetically modified mice carrying engrafted human tissues provide useful models to study human cell biology in physiologically relevant contexts. However, there remain several obstacles limiting the compatibility of human cells within their mouse hosts. Among these is inadequate cross-reactvitiy between certain mouse cytokines and human cellular receptors, depriving the graft of important survival and growth signals. To circumvent this problem, we utilized a lentivirus-based delivery system to express physiologically relevant levels of human interleukin-7 (hIL-7) in Rag2-/-γc-/- mice following a single intravenous injection. hIL-7 promoted homeostatic proliferation of both adoptively transferred and endogenously generated T-cells in Rag2-/-γc-/- Human Immune System (HIS) mice. Interestingly, we found that hIL-7 increased T lymphocyte numbers in the spleens of HIV infected HIS mice without affecting viral load. Taken together, our study unveils a versatile approach to deliver human cytokines to HIS mice, to both improve engraftment and determine the impact of cytokines on human diseases.
登录
查看更多内容
影响因子：
15.3
作者：
Bock, Thomas A.;Orlic, Donald;Dunbar, Cynthia E.;Broxmeyer, Hal E.;Bodine, David M.
通讯作者：
Bodine, David M.
影响因子：
56.9
作者：
Naldini, L;Blomer, U;Trono, D
通讯作者：
Trono, D
影响因子：
56.9
作者：
MCCUNE, JM;NAMIKAWA, R;WEISSMAN, IL
通讯作者：
WEISSMAN, IL
影响因子：
46.9
作者：
Yang, Lili;Yang, Haiguang;Wang, Pin
通讯作者：
Wang, Pin
影响因子：
30.3
作者：
Legrand N;Ploss A;Balling R;Becker PD;Borsotti C;Brezillon N;Debarry J;de Jong Y;Deng H;Di Santo JP;Eisenbarth S;Eynon E;Flavell RA;Guzman CA;Huntington ND;Kremsdorf D;Manns MP;Manz MG;Mention JJ;Ott M;Rathinam C;Rice CM;Rongvaux A;Stevens S;Spits H;Strick-Marchand H;Takizawa H;van Lent AU;Wang C;Weijer K;Willinger T;Ziegler P
通讯作者：
Ziegler P