Aedes aegypti Molecular Responses to Zika Virus: Modulation of Infection by the Toll and Jak/Stat Immune Pathways and Virus Host Factors.
Aedes aegypti Molecular Responses to Zika Virus: Modulation of Infection by the Toll and Jak/Stat Immune Pathways and Virus Host Factors.
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DOI：
10.3389/fmicb.2017.02050
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发表时间：
2017
影响因子：
5.2
通讯作者：
Dimopoulos G
中科院分区：
文献类型：
--
作者：
Angleró-Rodríguez YI;MacLeod HJ;Kang S;Carlson JS;Jupatanakul N;Dimopoulos G
寨卡病毒（ZIKV）和登革热病毒（DENV）通过伊蚊传播给人类。然而，载体和ZIKV之间的分子相互作用仍然在很大程度上未被探索。在这项工作中，我们进一步研究了ZIKV在两种不同的埃及伊蚊菌株中的向性，并表明病毒感染动力学、组织迁移和对感染的易感性在蚊子菌株之间不同。我们还比较了ZIKV或DENV感染后的载体转录组变化，证明在病毒摄入后7天，蚊子的中肠感染响应性转录组的40%是病毒特异性的。受调节的基因包括蚊子抗病毒免疫的关键因素。将ZIKV和DENV感染响应转录组数据与黄热病病毒和西尼罗河病毒的数据进行比较，确定了26个可能在伊蚊病毒感染中发挥关键作用的基因。通过反向遗传分析，我们表明Toll和Jak/Stat先天免疫途径介导对ZIKV感染的抗性增加，并且保守的DENV宿主因子vATPase和肌苷-5 ′-单磷酸脱氢酶也用于ZIKV感染。
Zika (ZIKV) and dengue virus (DENV) are transmitted to humans by Aedes mosquitoes. However, the molecular interactions between the vector and ZIKV remain largely unexplored. In this work, we further investigated the tropism of ZIKV in two different Aedes aegypti strains and show that the virus infection kinetics, tissue migration, and susceptibility to infection differ between mosquito strains. We also compare the vector transcriptome changes upon ZIKV or DENV infection demonstrating that 40% of the mosquito’s midgut infection-responsive transcriptome is virus-specific at 7 days after virus ingestion. Regulated genes included key factors of the mosquito’s anti-viral immunity. Comparison of the ZIKV and DENV infection-responsive transcriptome data to those available for yellow fever virus and West Nile virus identified 26 genes likely to play key roles in virus infection of Aedes mosquitoes. Through reverse genetic analyses, we show that the Toll and the Jak/Stat innate immune pathways mediate increased resistance to ZIKV infection, and the conserved DENV host factors vATPase and inosine-5′-monophosphate dehydrogenase are also utilized for ZIKV infection.
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影响因子：
2.2
作者：
Brackney, Doug E.;Isoe, Jun;Black, W. C., IV;Zamora, Jorge;Foy, Brian D.;Miesfeld, Roger L.;Olson, Ken E.
通讯作者：
Olson, Ken E.
影响因子：
3.8
作者：
Adelman, Zach N.;Anderson, Michelle A. E.;Myles, Kevin M.
通讯作者：
Myles, Kevin M.
影响因子：
6.7
作者：
Dong Y;Aguilar R;Xi Z;Warr E;Mongin E;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
3.8
作者：
Carvalho DO;McKemey AR;Garziera L;Lacroix R;Donnelly CA;Alphey L;Malavasi A;Capurro ML
通讯作者：
Capurro ML
影响因子：
5.3
作者：
Clayton AM;Dong Y;Dimopoulos G
通讯作者：
Dimopoulos G