Postfabrication Tethering of Molecular Gradients on Aligned Nanofibers of Functional Poly(ε-caprolactone)s.
Postfabrication Tethering of Molecular Gradients on Aligned Nanofibers of Functional Poly(ε-caprolactone)s.
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DOI：
10.1021/acs.biomac.9b01264
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发表时间：
2019-12-09
影响因子：
6.2
通讯作者：
Becker ML
中科院分区：
文献类型：
--
作者：
Silantyeva EA;Willits RK;Becker ML
结合地形和生化线索的底物对许多基础生物学研究非常有用。在包括细胞迁移在内的许多生物医学应用中，系留分子浓度梯度是非常需要的。在此，我们报告了一种用于沿纤维方向可调谐浓度梯度的排列纳米纤维衬底的制造的通用方法。以4-二苯并环辛醇（DIBO）为引发剂，对ε-己内酯（εCL）和烯丙基功能化的ε-己内酯（a ε pcl）进行开环共聚反应，得到了具有正交官能团柄的聚合物。通过接触纺丝将这些材料制成排列的纳米纤维衬底。在纺丝后，通过掩膜进行光活化巯基反应，用荧光标记染料的浓度梯度对纤维进行改性。作为证明，细胞粘附肽RGD通过菌株促进叠氮化物-炔环加成（SPAAC）在纤维表面的第二个功能化位点上化学连接。这种新方法提供了双功能纳米纤维衬底的制造，可以潜在地用于细胞迁移优化。
Substrates with combinations of topographical and biochemical cues are highly useful for a number of fundamental biological investigations. Tethered molecular concentration gradients in particular are highly desired for a number of biomedical applications including cell migration. Herein, we report a versatile method for the fabrication of aligned nanofiber substrates with a tunable concentration gradient along the fiber direction. 4-dibenzocyclooctynol (DIBO) was used as an initiator for the ring-opening copolymerization of ε-caprolactone (εCL) and allyl-functionalized ε-caprolactone (AεPCL) which yielded a well-defined polymer with orthogonal functional handles. These materials were fabricated into aligned nanofiber substrates via touch-spinning. Fibers were modified post-spinning with a concentration gradient of fluorescently labeled dye via a light activated thiol-ene reaction through a mask. As a demonstration, the cell adhesive peptide RGD was chemically tethered to the fiber surface at a second functionalization site via strain-promoted azide-alkyne cycloaddition (SPAAC). This novel approach affords fabrication of dual functional nanofiber substrates that can be potentially used for cell migration optimization.
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影响因子：
16.6
作者：
Just-Baringo, Xavier;Clark, Jemma;Gutmann, Matthias J.;Procter, David J.
通讯作者：
Procter, David J.
影响因子：
14
作者：
Callahan, Laura A. Smith;Xie, Sibai;Becker, Matthew L.
通讯作者：
Becker, Matthew L.
影响因子：
16.6
作者：
Ning, Xinghai;Guo, Jun;Boons, Geert-Jan
通讯作者：
Boons, Geert-Jan
影响因子：
4.8
作者：
Cavanaugh, McKay;Silantyeva, Elena;Becker, Matthew L.
通讯作者：
Becker, Matthew L.
影响因子：
5.2
作者：
de Almeida, Gabriela;Townsend, Lisa C.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.