Natural genetic variation drives microbiome selection in the Caenorhabditis elegans gut.
Natural genetic variation drives microbiome selection in the Caenorhabditis elegans gut.
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DOI：
10.1016/j.cub.2021.04.046
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发表时间：
2021-06-21
期刊：
影响因子：
--
通讯作者：
Samuel BS
中科院分区：
文献类型：
--
作者：
Zhang F;Weckhorst JL;Assié A;Hosea C;Ayoub CA;Khodakova AS;Cabrera ML;Vidal Vilchis D;Félix MA;Samuel BS
宿主遗传景观可以通过促进建立不同的生理环境来塑造动物肠道中的微生物组组装。然而，导致这些微生物组类型稳定性和变异的遗传决定因素在很大程度上仍然不确定。在这里，我们使用自由生活的线虫秀丽隐杆线虫，以确定自然的遗传变异之间的野生菌株的C。驱动不同微生物组组装的秀丽线虫菌株。为了实现这一目标，我们首先建立了一个多样化的模型微生物组，代表了C.野生的秀丽线虫利用这个社区，我们表明，C。秀丽线虫利用免疫、异生物质和代谢信号传导途径来促进不同微生物组类型的组装。这些途径的变化与特定细菌的富集有关，包括Alphaproteobacteria Ochrobactrum。利用RNAi和突变株，我们表明，宿主选择苍白杆菌是由宿主胰岛素信号通路特异性介导的。苍白杆菌的募集在缺乏IGFR-2/IGFR的情况下是钝的，并且通过胰岛素信号传导转录因子IGFR-16/FOXO和PQM-1/SALL 2的竞争作用来调节。此外，C.在成年时，线虫富集苍白杆菌与更快的动物生长速率和发育结束时更大的体型有关。这些结果强调了高度保守的胰岛素信号通路在C.优雅
Host genetic landscapes can shape microbiome assembly in the animal gut by contributing to the establishment of distinct physiological environments. However, the genetic determinants contributing to the stability and variation of these microbiome types remain largely undefined. Here, we use the free-living nematode Caenorhabditis elegans to identify natural genetic variation among wild strains of C. elegans strains that drives assembly of distinct microbiomes. To achieve this, we first established a diverse model microbiome that represents the strain-level phylogenetic diversity naturally encountered by C. elegans in the wild. Using this community, we show that C. elegans utilizes immune, xenobiotic and metabolic signaling pathways to favor the assembly of different microbiome types. Variations in these pathways were associated with enrichment for specific commensals, including the Alphaproteobacteria Ochrobactrum. Using RNAi and mutant strains, we showed that host selection for Ochrobactrum is mediated specifically by host insulin signaling pathways. Ochrobactrum recruitment is blunted in the absence of DAF-2/IGFR and modulated by the competitive action of insulin signaling transcription factors DAF-16/FOXO and PQM-1/SALL2. Further, the ability of C. elegans to enrich for Ochrobactrum as adults is correlated with faster animal growth rates and larger body size at the end of development. These results highlight a new role for the highly conserved insulin signaling pathways in the regulation of gut microbiome composition in C. elegans.
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影响因子：
30.8
作者：
Andersen, Erik C.;Gerke, Justin P.;Shapiro, Joshua A.;Crissman, Jonathan R.;Ghosh, Rajarshi;Bloom, Joshua S.;Felix, Marie-Anne;Kruglyak, Leonid
通讯作者：
Kruglyak, Leonid
影响因子：
10.5
作者：
Dowen RH;Breen PC;Tullius T;Conery AL;Ruvkun G
通讯作者：
Ruvkun G
影响因子：
3.7
作者：
Engelmann I;Griffon A;Tichit L;Montañana-Sanchis F;Wang G;Reinke V;Waterston RH;Hillier LW;Ewbank JJ
通讯作者：
Ewbank JJ
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura