Asymmetric distribution of a fluorescent sterol in synaptic plasma membranes: effects of chronic ethanol consumption.
Asymmetric distribution of a fluorescent sterol in synaptic plasma membranes: effects of chronic ethanol consumption.
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突触质膜中荧光甾醇的不对称分布：长期乙醇消耗的影响。
DOI：
10.1016/0005-2736(90)90103-u
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Nemecz,G
中科院分区：
文献类型：
--
作者：
Wood,WG;Schroeder,F;Hogy,L;Rao,AM;Nemecz,G
在一些研究中，乙醇诱导的膜结构变化归因于总膜胆固醇的增加。然而，在酒精消耗动物和酒精中毒患者的细胞膜中并未观察到胆固醇含量的一致变化。本研究探讨了假设，胆固醇是不对称分布在突触质膜（SPM）和慢性乙醇消耗改变跨双层分布的胆固醇。脱氢麦角固醇，一种荧光胆固醇类似物，用于检查长期乙醇处理和配对喂养对照组中的固醇分布和交换。细胞面小叶被发现有显着更多的脱氢麦角甾醇相比，外面小叶。这种不对称的分布显着减少慢性乙醇消耗是固醇运输。总胆固醇含量在两组之间没有差异。慢性乙醇消耗似乎改变transbilayer甾醇分布所确定的脱氢麦角甾醇在SPM中的掺入和分布。跨双层固醇分布的变化与最近关于乙醇在体外的不对称效应的报道一致（（1988）Biochim. Biophys. Acta 946，85-94）和体内（（（1989）J. Neurochem. 52，1925-1930）对膜小叶结构的研究。这项研究的结果也强调了除了不同脂质的总含量外，检查膜脂质结构域的重要性。
Ethanol-induced structural changes in membranes have in some studies been attributed to an increase in total membrane cholesterol. Consistent changes in cholesterol content, however, have not been observed in membranes of ethanol consuming animals and alcoholic patients. This study examined the hypotheses that cholesterol was asymmetrically distributed in synaptic plasma membranes (SPM) and that chronic ethanol consumption alters the transbilayer distribution of cholesterol. Dehydroergosterol, a fluorescent cholesterol analogue was used to examine sterol distribution and exchange in chronic ethanol-treated and pair-fed control groups. The cytofacial leaflet was found to have significantly more dehydroergosterol as compared to the exofacial leaflet. This asymmetric distribution was significantly reduced by chronic ethanol consumption as was sterol transport. Total cholesterol content did not differ between the two groups. Chronic ethanol consumption appeared to alter transbilayer sterol distribution as determined by the incorporation and distribution of dehydroergosterol in SPM. The changes in transbilayer sterol distribution are consistent with recent reports on the asymmetric effects of ethanol in vitro ((1988) Biochim. Biophys. Acta 946, 85–94) and in vivo ((1989) J. Neurochem. 52, 1925–1930) on membrane leaflet structure. The results of this study also underscore the importance of examining membrane lipid domains in addition to the total content of different lipids.
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DOI：
10.1016/0005-2736(85)90248-2
发表时间：
1985
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Rowe,ES
通讯作者：
Rowe,ES
一种快速、特异的血清总胆固醇超微量方法。
影响因子：
9.3
作者：
Robert E. Bowman;Richard C. Wolf
通讯作者：
Richard C. Wolf
使用荧光甾醇探测生物膜中甾醇的跨双层分布
影响因子：
3.5
作者：
F. Schroeder
通讯作者：
F. Schroeder
乙醇的膜效应：大量脂质与脂质域。
影响因子：
6.1
作者：
Wood,WG;Schroeder,F
通讯作者：
Schroeder,F
通过脱氢麦角甾醇的荧光猝灭确定甾醇跨质膜的不对称跨双层分布。
DOI：
10.1111/j.1432-1033.1982.tb06488.x
发表时间：
2005
期刊：
European journal of biochemistry
影响因子：
--
作者：
J. Hale;F. Schroeder
通讯作者：
F. Schroeder