Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland.
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland.
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DOI：
10.1371/journal.pone.0252743
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Wilson RM
中科院分区：
文献类型：
--
作者：
Cory AB;Chanton JP;Spencer RGM;Ogles OC;Rich VI;McCalley CK;IsoGenie Project Coordinators;EMERGE 2021 Field Team;Wilson RM
富含泥炭的泥炭地(“泥炭沼泽”)的碳(C)矿化速率异常缓慢的控制机制尚未完全了解，尽管对这一主题进行了数十年的研究。由于可溶性酚类化合物对缓慢的微生物代谢和细胞生长的亲和力，它们被认为是沼泽泥炭抵抗的潜在重要贡献者。尽管有这种潜在的重要作用，但可溶性酚类化合物对沼泽泥炭C矿化的影响尚不清楚。我们通过使用水溶性聚乙烯吡咯烷酮(PVP)控制厌氧沼泽和沼泽泥炭培养中游离酚的浓度来分析这种影响，PVP是一种与酚结合并使其失活的化合物，可以防止酚-酶相互作用。CO2和CH4的产生率(厌氧碳矿化的最终产物)通常与Michaelis-Menten(M.M.)饱和函数。使用M.M.参数，我们估计酚类物质抑制厌氧二氧化碳产生的程度在沼泽地区-62±16%-显著高于沼泽地区-14±4%。在甲烷产生方面，这种差异更为显著--其中对沼泽的酚类抑制率估计为54±19%，而沼泽没有表现出明显的抑制作用。与这种生境差异相一致，我们观察到沼泽比沼泽孔隙水中的可溶性酚类含量显著更高。综上所述，这些发现表明，可溶性酚可能导致沼泽异常顽强，以及(相对于其他泥炭地栖息地)较高的二氧化碳：甲烷产生率。
The mechanisms controlling the extraordinarily slow carbon (C) mineralization rates characteristic of Sphagnum-rich peatlands (“bogs”) are not fully understood, despite decades of research on this topic. Soluble phenolic compounds have been invoked as potentially significant contributors to bog peat recalcitrance due to their affinity to slow microbial metabolism and cell growth. Despite this potentially significant role, the effects of soluble phenolic compounds on bog peat C mineralization remain unclear. We analyzed this effect by manipulating the concentration of free soluble phenolics in anaerobic bog and fen peat incubations using water-soluble polyvinylpyrrolidone (“PVP”), a compound that binds with and inactivates phenolics, preventing phenolic-enzyme interactions. CO2 and CH4 production rates (end-products of anaerobic C mineralization) generally correlated positively with PVP concentration following Michaelis-Menten (M.M.) saturation functions. Using M.M. parameters, we estimated that the extent to which phenolics inhibit anaerobic CO2 production was significantly higher in the bog—62 ± 16%—than the fen—14 ± 4%. This difference was found to be more substantial with regards to methane production—wherein phenolic inhibition for the bog was estimated at 54 ± 19%, while the fen demonstrated no apparent inhibition. Consistent with this habitat difference, we observed significantly higher soluble phenolic content in bog vs. fen pore-water. Together, these findings suggest that soluble phenolics could contribute to bogs’ extraordinary recalcitrance and high (relative to other peatland habitats) CO2:CH4 production ratios.
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影响因子：
4.1
作者：
Berlin, Alex;Balakshin, Mikhail;Saddler, Jack
通讯作者：
Saddler, Jack
DOI：
10.1111/j.1365-2672.1990.tb01552.x
发表时间：
1990-10-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
GILBERT, P;PEMBERTON, D;WILKINSON, DE
通讯作者：
WILKINSON, DE
影响因子：
9.8
作者：
Brouns, Karlijn;Verhoeven, Jos T. A.;Hefting, Mariet M.
通讯作者：
Hefting, Mariet M.
影响因子：
4
作者：
Hodgkins, Suzanne B.;Chanton, Jeffrey P.;Cooper, William T.
通讯作者：
Cooper, William T.
影响因子：
16.6
作者：
Hodgkins SB;Richardson CJ;Dommain R;Wang H;Glaser PH;Verbeke B;Winkler BR;Cobb AR;Rich VI;Missilmani M;Flanagan N;Ho M;Hoyt AM;Harvey CF;Vining SR;Hough MA;Moore TR;Richard PJH;De La Cruz FB;Toufaily J;Hamdan R;Cooper WT;Chanton JP
通讯作者：
Chanton JP