Germ cells are not essential for sexual dimorphism of gonads in common carp, C. carpio L.
Germ cells are not essential for sexual dimorphism of gonads in common carp, C. carpio L.
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生殖细胞对于鲤鱼 (C. carpio L) 性腺的性二态性不是必需的。
DOI：
10.1016/j.aquaculture.2021.737501
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发表时间：
2022-01
期刊：
影响因子：
4.5
通讯作者：
Wei Hu
中科院分区：
文献类型：
--
作者：
Binbin Tao;Xianyao Liao;Lu Chen;Yongming Li;Kuangxin Chen;Shaoting Jia;Xingxing Wu;Wenge Ma;Yunya Wu;Wenrong Zhong;Ji Chen;Yanlong Song;Wei Hu
鲤鱼是世界上第四大养殖鱼类。鲤鱼的性二型生长使其性别分化成为重要的研究课题。本研究的主要目的是探讨生殖细胞在鲤鱼性别分化中的作用。我们在鲤鱼胚胎发育过程中，用吗啉代反义寡核苷酸敲除死亡端基因，获得了生殖细胞缺陷型鲤鱼。在无生殖细胞的鲤鱼中，我们发现大约一半的性腺（I型性腺）含有颗粒细胞和卵泡膜细胞，这与我们在野生型卵巢中观察到的体细胞相同。其余一半的性腺（II型性腺）含有支持细胞和间质细胞，这与野生型睾丸中的体细胞相同。此外，在无生殖细胞的性腺中也检测到性分化相关基因的表达。结果表明，cyp19a1a主要在I型性腺中表达，且各I型性腺中cyp19a1amRNA的表达量明显高于II型性腺。而dmrt 1主要在II型性腺中表达，且各II型性腺中dmrt 1mRNA的表达量均明显高于I型性腺。综合分析表明，生殖细胞缺陷鲤的性腺结构和性别分化相关基因的表达具有性二态性。这些发现对于揭示鲤鱼性别分化的内在机制具有重要意义，而这对于培育单性鲤鱼具有重要意义。
Common carp is the fourth most cultivated fish species in the world. Sexually dimorphic growth of common carp makes its sex differentiation an important research topic. The main objective of this study was to investigate the role of germ cells in sex differentiation of common carp. We generated the germ cell-deficient common carp through knocking downdead endgene with a morpholino antisense oligonucleotide during the embryogenesis. In the common carp without germ cells, we found about half of the gonads (type I gonads) contain granulosa cells and theca cells, which were the same with somatic cells we observed in the wild-type ovaries. The remaining half of the gonads (type II gonads) contain Sertoli cells and Leydig cells, which were the same with somatic cells in the wild-type testes. Furthermore, the expression of sex differentiation related genes was detected in the gonads without germ cells. It was foundcyp19a1awas mainly expressed in the type I gonads, and thecyp19a1amRNA level is much higher in each type I gonad than in the type II gonad. Whiledmrt1was mainly expressed in the type II gonads, and thedmrt1mRNA level is much higher in each type II gonad than in the type I gonad. Our comprehensive analysis showed that the gonadal structure and expression of genes related with sex differentiation were sexually dimorphic in the germ cell-deficient carp. These findings are valuable for discovering the underlying mechanism of common carp sex differentiation which is crucial for cultivating monosexual carp for aquaculture.
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影响因子：
4.5
作者：
Nishimura T;Yamada K;Fujimori C;Kikuchi M;Kawasaki T;Siegfried KR;Sakai N;Tanaka M
通讯作者：
Tanaka M
影响因子：
4.5
作者：
C. Biswas;S. Chakraborty;S. Munilkumar;P. Gireesh-Babu;P. Sawant;N. K. Chadha;G. Krishna;S. Dasgupta
通讯作者：
C. Biswas;S. Chakraborty;S. Munilkumar;P. Gireesh-Babu;P. Sawant;N. K. Chadha;G. Krishna;S. Dasgupta
影响因子：
2.7
作者：
Bin Tang;Wei Hu;Jun Hao;Zuoyan Zhu
通讯作者：
Bin Tang;Wei Hu;Jun Hao;Zuoyan Zhu
影响因子：
2.7
作者：
Dranow, Daniel B.;Tucker, Richard P.;Draper, Bruce W.
通讯作者：
Draper, Bruce W.
DOI：
10.1073/pnas.0611379104
发表时间：
2007-06-05
影响因子：
11.1
作者：
Morinaga, Chikako;Saito, Daisuke;Kondoh, Hisato
通讯作者：
Kondoh, Hisato