Germ cells in the teleost fish medaka have an inherent feminizing effect.
Germ cells in the teleost fish medaka have an inherent feminizing effect.
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DOI：
10.1371/journal.pgen.1007259
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发表时间：
2018-03
期刊：
影响因子：
4.5
通讯作者：
Tanaka M
中科院分区：
文献类型：
--
作者：
Nishimura T;Yamada K;Fujimori C;Kikuchi M;Kawasaki T;Siegfried KR;Sakai N;Tanaka M
生殖细胞产生卵子或精子。然而，最近对青鳉（Oryzias latipes）的分析表明，生殖细胞对于性腺的女性化也很重要，尽管生殖细胞的这种新作用尚未得到详细描述。在这里，我们证明女性化效应是生殖细胞固有的，不受配子发生阶段或生殖细胞的性命运的影响。产生了三种青鳉突变体来证明这种效果：figlα突变体，其中卵泡形成被破坏； meioC 突变体，其中生殖细胞无法进行配子发生和减数分裂；和dazl突变体，其中生殖细胞不发育成生殖母细胞。 XX突变体中所有这些不同阶段的生殖细胞都有能力使性腺女性化，从而形成具有卵巢结构的性腺。除了正常的卵巢发育外，我们还认为，性母细胞数量的增加足以使 XY 青鳉的性别逆转。这些结果可能从遗传学上证明，生殖细胞女性化作用的机制在生殖细胞的性命运决定和减数分裂之前被激活，可能在青鳉的生殖母细胞形成时被激活。生殖细胞是唯一可以通过精子或卵子将遗传物质转移到下一代的细胞。然而，最近对硬骨鱼的分析揭示了生殖细胞的另一个重要作用：使性腺女性化。在我们的研究中，配子发生在特定阶段被阻断的青鳉突变体提供了新的观点，即生殖细胞的女性化效应与其他生殖因素并行发生，例如减数分裂、生殖细胞的性命运决定和配子发生。青鳉的生殖细胞在发育时可能具有使性腺女性化的潜力。
Germ cells give rise to eggs or sperm. However, recent analyses in medaka (Oryzias latipes) showed that germ cells are also important for feminization of gonads, although this novel role of germ cells has not been characterized in detail. Here, we show that the feminizing effect is inherent to germ cells and is not affected by gametogenic stages or the sexual fate of germ cells. Three medaka mutants were generated to demonstrate this effect: figlα mutants, in which follicle formation is disrupted; meioC mutants, in which germ cells are unable to commit to gametogenesis and meiosis; and dazl mutants, in which germ cells do not develop into gonocytes. All these different stages of germ cells in XX mutants have an ability to feminize the gonads, resulting in the formation of gonads with ovarian structures. In addition to normal ovarian development, we also suggest that the increased number of gonocytes is sufficient for male to female sex reversal in XY medaka. These results may genetically demonstrate that the mechanism underlying the feminizing effect of germ cells is activated before the sexual fate decision of germ cells and meiosis, probably by the time of gonocyte formation in medaka. Germ cells are the only cells that can transfer genetic materials to the next generation via the sperm or egg. However, recent analyses in teleosts revealed another essential role of germ cells: feminizing the gonads. In our study, medaka mutants in which gametogenesis was blocked at specific stages provides the novel view that the feminizing effect of germ cells occurs in parallel with other reproductive elements, such as meiosis, the sexual fate decision of germ cells, and gametogenesis. Germ cells in medaka may have a potential to feminize gonads at the moment they have developed.
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作者：
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DOI：
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发表时间：
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影响因子：
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DOI：
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发表时间：
2015-11-06
影响因子：
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