Micromechanical regulation in cardiac myocytes and fibroblasts: implications for tissue remodeling.
Micromechanical regulation in cardiac myocytes and fibroblasts: implications for tissue remodeling.
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DOI：
10.1007/s00424-011-0931-8
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发表时间：
2011-07
影响因子：
4.5
通讯作者：
Russell, Brenda
中科院分区：
文献类型：
--
作者：
Curtis, Matthew W.;Russell, Brenda
心肌细胞在体内最具机械动力学的环境中安家。在细胞水平，室填充和排空的脉动刺激经历为循环应变（相对变形）和应力（每单位面积的力）。因此，产生张力的肌细胞和成纤维细胞的内在特征与其外在环境有着持续的机械相互作用。这篇综述探讨了如何在单个心肌细胞和成纤维细胞的规模的微观力学已被测量，建模，并在适应的背景下，在体外重演。这两种类型的心肌细胞响应外部施加的应变，和许多细胞内的mechanosensing途径已被确定与实验变量的仔细操纵。除应变外，肌细胞和成纤维细胞中的负载程度还受来自微环境的线索（如基质表面化学、硬度和形貌）的调节。需要在3D中组合这些结构线索来模拟来自发育、健康或病理生理心脏的细胞外基质的微机械复杂性。因此，对心脏细胞微观力学的理解可以为组织工程支架或干细胞壁龛的设计和组成提供信息，以供将来在再生医学中的应用。
Cells of the myocardium are at home in one of the most mechanically dynamic environments in the body. At the cellular level, pulsatile stimuli of chamber filling and emptying are experienced as cyclic strains (relative deformation) and stresses (force per unit area). The intrinsic characteristics of tension-generating myocytes and fibroblasts thus have a continuous mechanical interplay with their extrinsic surroundings. This review explores the ways that the micromechanics at the scale of single cardiac myocytes and fibroblasts have been measured, modeled, and recapitulated in vitro in the context of adaptation. Both types of cardiac cells respond to externally applied strain, and many of the intracellular mechanosensing pathways have been identified with the careful manipulation of experimental variables. In addition to strain, the extent of loading in myocytes and fibroblasts is also regulated by cues from the microenvironment such as substrate surface chemistry, stiffness, and topography. Combinations of these structural cues in 3D are needed to mimic the micromechanical complexity derived from the extracellular matrix of the developing, healthy, or pathophysiologic heart. An understanding of cardiac cell micromechanics can therefore inform the design and composition of tissue engineering scaffolds or stem cell niches for future applications in regenerative medicine.
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影响因子：
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作者：
Bray, Mark-Anthony;Sheehy, Sean P.;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
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作者：
Cupps BP;Taggar AK;Reynolds LM;Lawton JS;Pasque MK
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Pasque MK
影响因子：
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作者：
Amundsen, Brage H.;Crosby, Jonas;Stoylen, Asbjorn
通讯作者：
Stoylen, Asbjorn
DOI：
10.1152/ajpheart.2001.280.5.h2222
发表时间：
2001-05-01
影响因子：
4.8
作者：
Arts, T;Costa, KD;McCulloch, AD
通讯作者：
McCulloch, AD
DOI：
10.1083/jcb.153.2.413
发表时间：
2001-04-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bang ML;Mudry RE;McElhinny AS;Trombitás K;Geach AJ;Yamasaki R;Sorimachi H;Granzier H;Gregorio CC;Labeit S
通讯作者：
Labeit S