Effect of Photofrin-mediated photocytotoxicity on a panel of human pancreatic cancer cells.
Effect of Photofrin-mediated photocytotoxicity on a panel of human pancreatic cancer cells.
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DOI：
10.1016/j.pdpdt.2012.11.001
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发表时间：
2013-09
影响因子：
3.3
通讯作者：
Liu, Bolin
中科院分区：
文献类型：
--
作者：
Wang, Luo-Wei;Huang, Zheng;Lin, Han;Li, Zhao-Shen;Hetzel, Fred;Liu, Bolin
胰腺癌是男性和女性癌症相关死亡的主要原因。早期临床研究表明，光动力疗法（PDT）可能是治疗这种致命疾病的有效方法。在本研究中，检测了 Photofrin 介导的 PDT 对不同人胰腺癌细胞（BxPc-3、HPAF-II、Mia PaCa-2、MPanc-96、PANC-1 和 PL-45）的光细胞毒性。将癌细胞与 Photofrin (0—10 [H9262]g/ml) 共孵育 4 小时后，用 0—6 J/cm2 的 630 nm 光照射细胞。使用基于四唑的比色测定和克隆形成测定检查了 Photofrin PDT 对细胞存活的影响。通过流式细胞术分析PDT诱导的细胞凋亡。通过蛋白质印迹分析测定凋亡相关蛋白的表达。 Photofrin PDT 强烈抑制胰腺癌细胞的存活。 PDT LD50 后 24 小时，一小部分细胞 (<15%) 发生凋亡。 PDT 后 caspase-3、caspase-8、caspase-9 和 PARP 的裂解也得到证实。 BxPc-3、Mia PaCa-2、MPanc-96 和 PANC-1 细胞较敏感，HPAF-II 和 PL-45 细胞较不敏感。 Photofrin PDT 可诱导人胰腺癌细胞凋亡并抑制其存活。
Pancreatic cancer is a leading cause of cancer-related deaths in men and women. Early clinical studies suggest that photodynamic therapy (PDT) might be a useful modality in the management of this deadly disease. In this study, the photocytotoxicity of Photofrin-mediated PDT on different human pancreatic cancer cells (BxPc-3, HPAF-II, Mia PaCa-2, MPanc-96, PANC-1 and PL-45) was examined. After co-incubating cancer cells with Photofrin (0—10 [H9262]g/ml) for 4 h, the cells were irradiated with 0—6 J/cm2 of 630 nm light. The effect of Photofrin PDT on the survival of cells were examined using tetrazolium-based colorimetric assay and clonogenic assay. PDT-induced apoptosis was analyzed by flow cytometry. Expressions of apoptosis-related proteins were determined by western blot analysis. Photofrin PDT strongly inhibited the survival of pancreatic cancer cells. A small portion of cells (<15%) underwent apoptosis 24 h after PDT at LD50. Cleavage of caspase-3, caspase-8, caspase-9 and PARP after PDT were also confirmed. BxPc-3, Mia PaCa-2, MPanc-96, and PANC-1 cells were more sensitive and HPAF-II and PL-45 cells less sensitive. Photofrin PDT can induce apoptosis and inhibit survival of human pancreatic cancer cells.
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影响因子：
3.3
作者：
Kessel, D;Luo, Y;Chang, CK
通讯作者：
Chang, CK
影响因子：
9.6
作者：
Hajri, A;Coffy, S;Aprahamian, M
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影响因子：
3.3
作者：
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Sibata, Claudio H.
影响因子：
3.3
作者：
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通讯作者：
WIEMAN, TJ
影响因子：
3.6
作者：
Ayaru, Lakshmana;Wittmann, Johannes;Pereira, Stephen P.
通讯作者：
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