Correlated conformational events in EF-G and the ribosome regulate translocation.
Correlated conformational events in EF-G and the ribosome regulate translocation.
复制标题
DOI：
10.1038/nsmb.1925
复制
发表时间：
2010-12
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
--
作者：
在细菌中，tRNA和mRNA相对于核糖体的转位是由保守的GTP酶，延伸因子-G(EF-G)催化的。为了探索这一过程中的速率决定特征，利用单分子荧光共振能量转移从两个独特的结构角度对EF-G催化的易位进行了成像。数据显示，核糖体自发达到瞬时“解锁”状态的速率与EF-G的tRNA样结构域IV/V元件与A位点的结合速率密切相关。在这些结构转变之后，转位发生得相对较快，这表明核糖体固有的构象过程决定了转位的速度。在非水解性GTP类似物和特定抗生素存在下进行的实验进一步表明，EF-G和核糖体中的变构连接构象事件介导了底物的快速定向移动和EF-G的释放。
In bacteria, the translocation of tRNA and mRNA with respect to the ribosome is catalyzed by the conserved GTPase, elongation factor-G (EF-G). In order to probe the rate determining features in this process, EF-G-catalyzed translocation was imaged from two unique structural perspectives using single-molecule fluorescence resonance energy transfer. The data reveal that the rate at which the ribosome spontaneously achieves a transient, “unlocked” state is closely correlated with the rate at which the tRNA-like, domain IV/V element of EF-G engages the A site. Following these structural transitions, translocation occurs comparatively fast, suggesting that conformational processes intrinsic to the ribosome determine the rate of translocation. Experiments performed in the presence of non-hydrolyzable GTP analogues and specific antibiotics further reveal that allosterically linked conformational events in EF-G and the ribosome mediate rapid, directional substrate movement and EF-G release.
登录
查看更多内容
影响因子：
6.1
作者：
Agirrezabala, Xabier;Frank, Joachim
通讯作者：
Frank, Joachim
影响因子：
14.8
作者：
Feldman MB;Terry DS;Altman RB;Blanchard SC
通讯作者：
Blanchard SC
影响因子：
3.4
作者：
Aitken, Colin Echeverria;Marshall, R. Andrew;Puglisi, Joseph D.
通讯作者：
Puglisi, Joseph D.
DOI：
10.1016/0006-291x(69)90934-6
发表时间：
1969-01-01
影响因子：
3.1
作者：
BODLEY, JW;ZIEVE, FJ;ZIEVE, ST
通讯作者：
ZIEVE, ST
影响因子：
3.4
作者：
Dave, Richa;Terry, Daniel S.;Blanchard, Scott C.
通讯作者：
Blanchard, Scott C.