The efficiency of design-based stereology in estimating spiral ganglion populations in mice.
The efficiency of design-based stereology in estimating spiral ganglion populations in mice.
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DOI：
10.1016/j.heares.2013.07.007
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发表时间：
2013-10
期刊：
影响因子：
2.8
通讯作者：
Lauer, Amanda M.
中科院分区：
文献类型：
--
作者：
Schettino, Amy E.;Lauer, Amanda M.
细胞群的准确定量在评估和评价实验组中的神经存活和退化中是必不可少的。通过传统的二维计数方法获得的估计值受到与待计数的神经元的大小和形状相关的计数参数的严重偏置，导致大范围的不准确计数。相比之下，计算群体中的每个细胞可能是非常劳动密集型的。本研究假设，基于设计的体视学提供了对小鼠耳蜗螺旋神经节神经元（SGN）总数的估计，该估计与通过其他准确细胞计数方法（例如连续重建）获得的估计相当，同时是一种更有效的方法。SGN对于将听觉信息从毛细胞传递到听觉脑干是必不可少的，并且调查影响其退化的因素可以深入了解听力功能障碍进展的生理基础。体视学定量技术提供了独立于SGN的大小和形状的有效采样的益处。使用光学分馏探针和传统的二维计数方法获得了听力正常的年轻C57小鼠和年龄相关性听力损失的C57小鼠耳蜗SGN的群体估计。正常听力小鼠中SGN的平均估计数量为7009，而年龄相关性听力损失小鼠中SGN的平均估计数量为5096。听力正常小鼠中SGN的估计数量在文献中先前报道的值范围内。年龄相关性听力损失动物的SGN群体减少具有统计学显著性。与二维方法相比，体视学测量每个切片所需的时间更少，同时优化了分析的耳蜗组织的量。这些发现表明，基于设计的体视学为其他计数方法提供了一种实用的替代方法，例如Abercrombie校正方法，该方法已被证明明显低估了细胞群，以及单独考虑每个细胞的劳动密集型方案。
Accurate quantification of cell populations is essential in assessing and evaluating neural survival and degeneration in experimental groups. Estimates obtained through traditional two-dimensional counting methods are heavily biased by the counting parameters in relation to the size and shape of the neurons to be counted, resulting in a large range of inaccurate counts. In contrast, counting every cell in a population can be extremely labor-intensive. The present study hypothesizes that design-based stereology provides estimates of the total number of cochlear spiral ganglion neurons (SGNs) in mice that are comparable to those obtained by other accurate cell-counting methods, such as a serial reconstruction, while being a more efficient method. SGNs are indispensable for relaying auditory information from hair cells to the auditory brainstem, and investigating factors affecting their degeneration provides insight into the physiological basis for the progression of hearing dysfunction. Stereological quantification techniques offer the benefits of efficient sampling that is independent of the size and shape of the SGNs. Population estimates of SGNs in cochleae from young C57 mice with normal-hearing and C57 mice with age-related hearing loss were obtained using the optical fractionator probe and traditional two-dimensional counting methods. The average estimated population of SGNs in normal-hearing mice was 7009, whereas the average estimated population in mice with age-related hearing loss was 5096. The estimated population of SGNs in normal-hearing mice fell within the range of values previously reported in the literature. The reduction in the SGN population in animals with age-related hearing loss was statistically significant. Stereological measurements required less time per section compared to two-dimensional methods while optimizing the amount of cochlear tissue analyzed. These findings demonstrate that design-based stereology provides a practical alternative to other counting methods such as the Abercrombie correction method, which has been shown to notably underestimate cell populations, and labor-intensive protocols that account for every cell individually.
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DOI：
10.1007/s101620020028
发表时间：
2002-09-01
期刊：
JARO
影响因子：
--
作者：
Wang, Y;Hirose, K;Liberman, MC
通讯作者：
Liberman, MC
影响因子：
3.3
作者：
Whitlon, DS;Ketels, KV;Richter, CP
通讯作者：
Richter, CP
DOI：
10.1046/j.1365-2818.2001.00958.x
发表时间：
2001-12-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
Dorph-Petersen, KA;Nyengaard, JR;Gundersen, HJG
通讯作者：
Gundersen, HJG
DOI：
10.1523/jneurosci.2845-09.2009
发表时间：
2009-11-11
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Kujawa SG;Liberman MC
通讯作者：
Liberman MC
影响因子：
4.6
作者：
Agerman, K;Hjerling-Leffler, J;Ernfors, P
通讯作者：
Ernfors, P