Genetic polymorphism of merozoite surface protein-1 in Plasmodium falciparum isolates from patients with mild to severe malaria in Libreville, Gabon.
Genetic polymorphism of merozoite surface protein-1 in Plasmodium falciparum isolates from patients with mild to severe malaria in Libreville, Gabon.
复制标题
DOI：
10.1051/parasite/2015012
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Mawili-Mboumba DP
中科院分区：
文献类型：
--
作者：
Bouyou-Akotet MK;M'Bondoukwé NP;Mawili-Mboumba DP
我们评估了疟原虫（P.）恶性疟原虫等位基因多样性的基础上的临床严重程度和年龄。该研究于2011年至2012年在加蓬利伯维尔进行，那里的疟疾患病率为24.5%。分析了合并症和非合并症疟疾患者分离株裂殖子表面蛋白1（msp 1）基因座的多态性。在滤纸上收集血液。DNA提取后，使用巢式PCR进行msp 1基因的基因分型。K1，Ro 33和Mad 20等位基因家族分别在71（63%），64（57%）和38（34%）的112个分析样本中检测到。总体上，检测到17个K1和11个Mad 20等位基因。msp 1等位基因家族与年龄无关。Mad 20等位基因多样性随着疟疾的严重程度而增加。K1和Mad 20等位基因数随年龄增长而减少。感染复数（MOI）为1-6种基因型，感染复杂度（COI）为1.8 ± 1. COI随年龄的不同而不同：成人分离株的COI为1.9（±1.1），0-5岁儿童分离株的COI为1.8（±1.1），而6-15岁儿童分离株的COI往往较低（1.6 ± 0.8）。在利伯维尔流行的恶性疟原虫菌株中发现了广泛的遗传多样性。特异性msp 1等位基因的数量随着临床严重程度的增加而增加，表明疟疾的多样性和严重程度之间存在关联。
We assessed Plasmodium (P.) falciparum allelic diversity based on clinical severity and age. The study was conducted from 2011 to 2012 in Libreville, Gabon where malaria prevalence was 24.5%. The polymorphism of the merozoite surface protein-1 (msp1) locus was analyzed in isolates from patients with complicated and uncomplicated malaria. Blood was collected on filter paper. After DNA extraction, genotyping of the msp1 gene was performed using nested PCR. The K1, Ro33, and Mad20 allelic families were detected in 71 (63%), 64 (57%), and 38 (34%) of the 112 analyzed samples, respectively. Overall, 17 K1 and 11 Mad20 alleles were detected. There was no association between msp1 allelic families and age. Mad20 allelic diversity increased with the severity of malaria. The number of K1 and Mad20 alleles decreased with age. The multiplicity of infection (MOI) was 1–6 genotypes and the complexity of infection (COI) 1.8 ± 1. The COI differed based on age: it was 1.9 (±1.1) in the isolates from adults, 1.8 (±1.1) in those from 0–5 year-old children, whereas it tended to be lower (1.6 ± 0.8) in those from 6–15 year-old children. Extensive genetic diversity is found in P. falciparum strains circulating in Libreville. The number of specific msp1 alleles increased with clinical severity, suggesting an association between the diversity and the severity of malaria.
登录
查看更多内容
影响因子：
2.7
作者：
Amodu, OK;Adeyemo, AA;Omotade, OO
通讯作者：
Omotade, OO
DOI：
10.1073/pnas.1120455109
发表时间：
2012-06-26
影响因子：
11.1
作者：
Lavstsen, Thomas;Turner, Louise;Theander, Thor G.
通讯作者：
Theander, Thor G.
影响因子：
5.8
作者：
Mayengue, Pembe Issarnou;Luty, Adrian J. F.;Ntoumi, Francine
通讯作者：
Ntoumi, Francine
影响因子：
6.4
作者：
Ariey, F;Hommel, D;Mercereau-Puijalon, O
通讯作者：
Mercereau-Puijalon, O
影响因子：
3.7
作者：
Bouyou-Akotet, Marielle Karine;Mawili-Mboumba, Denise Patricia;Kombila, Maryvonne
通讯作者：
Kombila, Maryvonne