Hypoxia promotes progesterone synthesis during luteinization in bovine granulosa cells.
Hypoxia promotes progesterone synthesis during luteinization in bovine granulosa cells.
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DOI：
10.1262/jrd.2014-014
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Okuda K
中科院分区：
文献类型：
--
作者：
Fadhillah;Yoshioka S;Nishimura R;Okuda K
为了确定缺氧是否对黄体化有影响，我们研究了缺氧对牛黄体化和非黄体化颗粒细胞培养模型的影响。颗粒细胞取自直径≤ 6 mm的小窦卵泡。为了诱导黄体化，在20% O2下用胰岛素（2 µg/ml）、毛喉素（10 µM）或胰岛素与毛喉素联合处理细胞24 h。24小时后，孕酮（P4）的生产是较高的处理细胞，我们定义为黄体化颗粒细胞，比未处理的细胞，我们定义为非黄体化颗粒细胞。P4生产的非黄体化颗粒细胞不受缺氧（24小时在10%和5%O2），而P4生产的颗粒细胞与胰岛素结合毛喉素处理的显着增加缺氧（24小时在10%和5%O2）。由于缺氧影响P4的产生的黄体化颗粒细胞，但不通过非黄体化颗粒细胞，缺氧似乎促进P4的生产过程中，而不是之前，黄体化。在胰岛素联合福司可林处理的细胞中，10%O2下类固醇生成急性调节蛋白（星星）和3β-羟基类固醇脱氢酶（3β-HSD）的mRNA和蛋白表达增加，而5%O2下P4生物合成关键蛋白和酶的mRNA和蛋白表达没有增加。结果提示，低氧通过促进星星和3β-HSD表达促进P4的生成，在黄体形成早期促进黄体化的进行和完成。
To determine whether hypoxia has an effect on luteinization, we examined the influence of hypoxia on a model of bovine luteinizing and non-luteinizing granulosa cell culture. The granulosa cells were obtained from small antral follicles (≤ 6 mm in diameter). To induce luteinization, the cells were treated for 24 h with insulin (2 µg/ml), forskolin (10 µM) or insulin in combination with forskolin at 20% O2. After 24 h, progesterone (P4) production was higher in the treated cells, which we defined as luteinizing granulosa cells, than in non-treated cells, which we defined as non-luteinizing granulosa cells. P4 production by non-luteinizing granulosa cells was not affected by hypoxia (24 h at 10% and 5% O2), while P4 production by granulosa cells treated with insulin in combination with forskolin was significantly increased under hypoxia (24 h at 10% and 5% O2). Because hypoxia affected P4 production by the luteinizing granulosa cells but not by the non-luteinizing granulosa cells, hypoxia seems to promote P4 production during, rather than before, luteinization. In the cells treated with insulin in combination with forskolin, mRNA and protein expression of steroidogenic acute regulatory protein (StAR) and protein expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) increased under 10% O2, while mRNA and protein expressions of key protein and enzymes in P4 biosynthesis did not increase under 5% O2. The overall results suggest that hypoxia plays a role in progressing and completing the luteinization by enhancing P4 production through StAR as well as 3β-HSD expressions in the early time of establishing the corpus luteum.
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影响因子：
1.8
作者：
Okuda, Kiyoshi;Uenoyama, Yoshihisa;Skarzynski, Dariusz Jan
通讯作者：
Skarzynski, Dariusz Jan
影响因子：
2.9
作者：
LABARCA, C;PAIGEN, K
通讯作者：
PAIGEN, K
影响因子：
3.6
作者：
CONCANNON, P;HANSEL, W;MCENTEE, K
通讯作者：
MCENTEE, K
影响因子：
4.8
作者：
Lioutas, C;Einspanier, A;Ivell, R
通讯作者：
Ivell, R
影响因子：
--
作者：
CLARK, BJ;SOO, SC;STOCCO, DM
通讯作者：
STOCCO, DM