Ecdysone receptor acts in fruitless- expressing neurons to mediate drosophila courtship behaviors.
Ecdysone receptor acts in fruitless- expressing neurons to mediate drosophila courtship behaviors.
复制标题
DOI：
10.1016/j.cub.2009.06.063
复制
发表时间：
2009-09-15
期刊：
影响因子：
--
通讯作者：
Arbeitman MN
中科院分区：
文献类型：
--
作者：
Dalton JE;Lebo MS;Sanders LE;Sun F;Arbeitman MN
在黑腹果蝇中，无果 (fru) 编码求偶行为所需的雄性特异性转录因子（FRUM；由 fru P1 编码）[已综述]。然而，FRUM 在整个发育过程中的下游效应器在很大程度上是未知的。在变态过程中，神经系统针对成年功能进行重塑，其时间由类固醇激素 20-羟基蜕皮激素（蜕皮激素）通过蜕皮激素受体（核受体 EcR 的异二聚体（异构体为 EcR-A、EcR-B1 或 EcR-B2）和 Ultraspiracle (USP) 协调）[已综述]。在这里，我们发现在变态过程中被确定为 FRUM 下游调节的基因明显高于已知响应蜕皮激素或 EcR 调节的基因。 FRUM 和 EcR 同工型在变态过程中以同工型特异性方式在 CNS 神经元中共表达。雄性表达 fru P1 的神经元中 EcR-A 水平的降低导致雄性之间的求爱活动显着增加，并且两个触角叶肾小球的大小显着减小。还鉴定出在表达 fru P1 的神经元中受 EcR-A 下游调节的其他基因。因此，表达 fru P1 的神经元需要 EcR-A 来实现野生型雄性求偶行为和雄性特异性神经元结构的建立。
In Drosophila melanogaster, fruitless (fru) encodes male-specific transcription factors (FRUM; encoded by fru P1) required for courtship behaviors [reviewed in ]. However, downstream effectors of FRUM throughout development are largely unknown. During metamorphosis the nervous system is remodeled for adult function, the timing of which is coordinated by the steroid hormone 20-hydroxy ecdysone (ecdysone) through the ecdysone receptor, a heterodimer of the nuclear receptors EcR (isoforms are EcR-A, EcR-B1, or EcR-B2) and Ultraspiracle (USP) [reviewed in ]. Here, we show that genes identified as regulated downstream of FRUM during metamorphosis are significantly overrepresented with genes known to be regulated in response to ecdysone or EcR. FRUM and EcR isoforms are co-expressed in neurons in the CNS during metamorphosis in an isoform-specific manner. Reduction of EcR-A levels in fru P1-expressing neurons of males caused a significant increase in male-male courtship activity and significant reduction in size of two antennal lobe glomeruli. Additional genes were identified that are regulated downstream of EcR-A in fru P1-expressing neurons. Thus, EcR-A is required in fru P1-expressing neurons for wild type male courtship behaviors and the establishment of male-specific neuronal architecture.
登录
查看更多内容
影响因子：
10.5
作者：
Dauwalder, B;Tsujimoto, S;Mattox, W
通讯作者：
Mattox, W
影响因子：
4.6
作者：
Cherbas, L;Hu, X;Cherbas, P
通讯作者：
Cherbas, P
影响因子：
4.6
作者：
Brown, HLD;Cherbas, L;Truman, JW
通讯作者：
Truman, JW
影响因子：
16.2
作者：
Kimura, Ken-ichi;Hachiya, Tomoaki;Yamamoto, Daisuke
通讯作者：
Yamamoto, Daisuke
影响因子：
4.5
作者：
Goldman TD;Arbeitman MN
通讯作者：
Arbeitman MN