Superior Colliculus GABAergic Neurons Are Essential for Acute Dark Induction of Wakefulness in Mice
Superior Colliculus GABAergic Neurons Are Essential for Acute Dark Induction of Wakefulness in Mice
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上丘 GABA 能神经元对于小鼠急性暗诱导觉醒至关重要
DOI：
10.1016/j.cub.2018.12.031
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发表时间：
2019-02
期刊：
影响因子：
9.2
通讯作者：
Huang Ahi-Li
中科院分区：
文献类型：
--
作者：
Zhang Ze;Liu Wen-Ying;Diao Yu-Pu;Xu Wei;Zhong Yu-Heng;Zhang Jia-Yi;Lazarus Michael;Liu Yuan-Yuan;Qu Wei-Min;Huang Ahi-Li
睡眠是由内稳态过程和生物钟调节的。光通过将生物钟带入太阳日来间接调节睡眠。光也可以独立于光诱导影响睡眠[1]。急性光照可以诱导睡眠，急性暗脉冲可以增加夜间动物的觉醒[1，2]。视网膜中介导光和暗对睡眠影响的光感受器和细胞类型得到了很好的表征[1-4]。一些研究探索了参与急性光诱导睡眠的大脑区域。Fos表达和非特异性病变表明，上级丘（SC）可能在急性光诱导睡眠中发挥作用[2，5]。相比之下，介导急性黑暗诱导觉醒的大脑区域和神经回路尚不清楚。本实验证明，视网膜神经节细胞（retinalganglioncells，RGC）对小鼠SC内GABA能神经元有直接的支配作用，并且这些细胞的活动受到急性暗脉冲的抑制，而不受光脉冲的影响。此外，消融SC GABA能神经元消除了急性黑暗诱导的觉醒，但不是光诱导的睡眠。在光遗传学和电生理实验的基础上，我们发现SC GABA能神经元与腹侧被盖区（VTA）的多巴胺能神经元形成单突触功能联系。选择性损害腹侧被盖区多巴胺能细胞完全取消急性黑暗诱导觉醒，而不影响睡眠的光诱导。总的来说，我们的研究结果揭示了视网膜-SC GABA能-VTA多巴胺能回路在急性黑暗诱导觉醒中的基本作用，并表明黑暗和光明信号通过不同的途径影响睡眠-觉醒行为。
Sleep is regulated by homeostatic process and circadian clock. Light indirectly modulates sleep by entraining the circadian clock to the solar day. Light can also influence sleep independent of photo-entrainment [1]. An acute light exposure could induce sleep, and an acute dark pulse could increase wakefulness in nocturnal animals [1, 2]. The photoreceptors and cell types in the retina that mediate light and dark effects on sleep are well characterized [1–4]. A few studies have explored the brain region involved in acute light induction of sleep. Fos expression and nonspecific lesions suggest that the superior colliculus (SC) may play a role in acute light induction of sleep [2, 5]. In contrast, the brain area and neural circuits mediating acute dark induction of wakefulness are unknown. Here, we demonstrated that retina ganglion cells (RGCs) had direct innervations on the GABAergic neurons in the mouse SC, and the activities of these cells were inhibited by an acute dark pulse, but not influenced by a light pulse. Moreover, ablating SC GABAergic neurons abolished the acute dark induction of wakefulness, but not light induction of sleep. Based on optogenetic and electrophysiological experiments, we found that SC GABAergic neurons formed monosynaptic functional connections with dopaminergic neurons in the ventral tegmental area (VTA). Selective lesions of VTA dopaminergic cells totally abolished acute dark induction of wakefulness without affecting the light induction of sleep. Collectively, our findings uncover a fundamental role for a retinal-SC GABAergic-VTA dopaminergic circuit in acute dark induction of wakefulness and indicate that the dark and light signals affect sleep-wake behaviors through distinct pathways.
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DOI：
10.1523/jneurosci.01-08-00876.1981
发表时间：
1981-08
期刊：
--
影响因子：
--
作者：
G. Aston-Jones;F. Bloom
通讯作者：
G. Aston-Jones;F. Bloom
DOI：
10.1523/jneurosci.2924-12.2013
发表时间：
2013-01-02
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
DesJardin JT;Holmes AL;Forcelli PA;Cole CE;Gale JT;Wellman LL;Gale K;Malkova L
通讯作者：
Malkova L
影响因子：
14.8
作者：
Zhang F;Gradinaru V;Adamantidis AR;Durand R;Airan RD;de Lecea L;Deisseroth K
通讯作者：
Deisseroth K
伏隔核通过表达多巴胺 D1 受体的神经元亚群控制觉醒
影响因子：
16.6
作者：
Luo YJ;Li YD;Wang L;Yang SR;Yuan XS;Wang J;Cherasse Y;Lazarus M;Chen JF;Qu WM;Huang ZL
通讯作者：
Huang ZL
影响因子：
3
作者：
Muindi F;Zeitzer JM;Heller HC
通讯作者：
Heller HC