Separate transcriptionally regulated pathways specify distinct classes of sister dendrites in a nociceptive neuron.
Separate transcriptionally regulated pathways specify distinct classes of sister dendrites in a nociceptive neuron.
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DOI：
10.1016/j.ydbio.2017.10.009
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发表时间：
2017-12-15
影响因子：
2.7
通讯作者：
Miller DM 3rd
中科院分区：
文献类型：
--
作者：
O'Brien BMJ;Palumbos SD;Novakovic M;Shang X;Sundararajan L;Miller DM 3rd
伤害性神经元的树突将外部信号转化为神经化学信号，提醒有机体注意潜在的破坏性刺激。每个感觉神经元的感受野是由它的树枝来定义的，但形成树突结构的机制还不完全清楚。利用线虫的PVD神经元模型伤害性感受器，我们确定了两种类型的PVD侧支沿着背轴/腹轴投射，以产生PVD树突：(1)附着在表皮上的先锋树突；(2)与周向运动神经元突起成束的连合树突。以往的报道表明，LIM同源结构域转录因子MEC-3是所有高阶PVD分支所必需的，其靶标之一，Claudin样膜蛋白HPO-30，优先促进先锋分支的生长。在这里，我们证明了另一个MEC-3靶点，保守的TFIIA样锌指转录因子EGL-46，采用了指定连合树突的替代角色。已知的EGL-46结合伙伴，Tead转录因子EGL-44，也是PVD连合支生长所必需的。HPO-30和EGL-44双突变体的侧枝缺陷明显增强，先锋树突和连合树突减少。因此，HPO-30/Claudin和EGL-46/EGL-44在MEC-3下游发挥作用，并平行作用于两种不同类型的PVD树突状分支的生长。
The dendritic processes of nociceptive neurons transduce external signals into neurochemical cues that alert the organism to potentially damaging stimuli. The receptive field for each sensory neuron is defined by its dendritic arbor, but the mechanisms that shape dendritic architecture are incompletely understood. Using the model nociceptor, the PVD neuron in C. elegans, we determined that two types of PVD lateral branches project along the dorsal/ventral axis to generate the PVD dendritic arbor: (1) Pioneer dendrites that adhere to the epidermis, and (2) Commissural dendrites that fasciculate with circumferential motor neuron processes. Previous reports have shown that the LIM homeodomain transcription factor MEC-3 is required for all higher order PVD branching and that one of its targets, the claudin-like membrane protein HPO-30, preferentially promotes outgrowth of pioneer branches. Here, we show that another MEC-3 target, the conserved TFIIA-like zinc finger transcription factor EGL-46, adopts the alternative role of specifying commissural dendrites. The known EGL-46 binding partner, the TEAD transcription factor EGL-44, is also required for PVD commissural branch outgrowth. Double mutants of hpo-30 and egl-44 show strong enhancement of the lateral branching defect with decreased numbers of both pioneer and commissural dendrites. Thus, HPO-30/Claudin and EGL-46/EGL-44 function downstream of MEC-3 and in parallel acting pathways to direct outgrowth of two distinct classes of PVD dendritic branches.
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DOI：
10.1111/j.1460-9568.2010.07447.x
发表时间：
2010-11
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Barry J;Gu Y;Gu C
通讯作者：
Gu C
影响因子：
2.4
作者：
Mohammadi A;Byrne Rodgers J;Kotera I;Ryu WS
通讯作者：
Ryu WS
影响因子：
2.7
作者：
Tsalik, EL;Niacaris, T;Hobert, O
通讯作者：
Hobert, O
影响因子：
25
作者：
通讯作者：
--
DOI：
10.1126/science.1189095
发表时间：
2010-06-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Oren-Suissa M;Hall DH;Treinin M;Shemer G;Podbilewicz B
通讯作者：
Podbilewicz B