Behavioral response of Caenorhabditis elegans to localized thermal stimuli.
Behavioral response of Caenorhabditis elegans to localized thermal stimuli.
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DOI：
10.1186/1471-2202-14-66
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发表时间：
2013-07-03
期刊：
影响因子：
2.4
通讯作者：
Ryu WS
中科院分区：
文献类型：
--
作者：
Mohammadi A;Byrne Rodgers J;Kotera I;Ryu WS
伤害感受会引起快速的退缩行为，旨在保护动物免受潜在危险。当头部或尾部分别受到有害刺激时，线虫会做出反射性逆转或向前运动反应。在这里，我们开发了一种对红外激光刺激进行精确空间和时间控制的检测方法，该刺激瞄准蠕虫身体的五分之一，并量化蠕虫逃避反应的多个方面。当头部受到刺激时，我们发现，小至摄氏度的温度变化就可以引发逃避反应，并且随着有害刺激幅度的增加，逃避行为的各个方面（例如反应潜伏期和逃避方向）会发生有利的变化。我们绘制了蠕虫整个身体的热伤害感受的行为感受野，并显示出与头部和尾部反应不同的中体回避行为。在身体中部，蠕虫对小至 80 μm 的刺激位置的变化都很敏感。这种中间体反应是概率性的，在刺激后产生向后、向前或暂停状态。当刺激位置分别从蠕虫的中部向前部或后部移动时，这些状态的分布从反向偏置变为前向偏置。我们使用钙成像、基因消融和激光消融将 PVD 确定为中体反应的热伤害感受器。对突变体的分析表明 TRPV 通道和谷氨酸可能参与促进中体毒性反应。通过高分辨率定量行为分析，我们全面表征了秀丽隐杆线虫对其身体施加的有害热刺激的逃避反应，并发现了一种新颖的中体反应。我们进一步确定了伤害感受器 PVD 需要感知身体中部的有害热量，并且可以在空间上区分局部热刺激。
Nociception evokes a rapid withdrawal behavior designed to protect the animal from potential danger. C. elegans performs a reflexive reversal or forward locomotory response when presented with noxious stimuli at the head or tail, respectively. Here, we have developed an assay with precise spatial and temporal control of an infrared laser stimulus that targets one-fifth of the worm’s body and quantifies multiple aspects of the worm’s escape response. When stimulated at the head, we found that the escape response can be elicited by changes in temperature as small as a fraction of a degree Celsius, and that aspects of the escape behavior such as the response latency and the escape direction change advantageously as the amplitude of the noxious stimulus increases. We have mapped the behavioral receptive field of thermal nociception along the entire body of the worm, and show a midbody avoidance behavior distinct from the head and tail responses. At the midbody, the worm is sensitive to a change in the stimulus location as small as 80 μm. This midbody response is probabilistic, producing either a backward, forward or pause state after the stimulus. The distribution of these states shifts from reverse-biased to forward-biased as the location of the stimulus moves from the middle towards the anterior or posterior of the worm, respectively. We identified PVD as the thermal nociceptor for the midbody response using calcium imaging, genetic ablation and laser ablation. Analyses of mutants suggest the possibility that TRPV channels and glutamate are involved in facilitating the midbody noxious response. Through high resolution quantitative behavioral analysis, we have comprehensively characterized the C. elegans escape response to noxious thermal stimuli applied along its body, and found a novel midbody response. We further identified the nociceptor PVD as required to sense noxious heat at the midbody and can spatially differentiate localized thermal stimuli.
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影响因子：
3.7
作者：
Liu S;Schulze E;Baumeister R
通讯作者：
Baumeister R
影响因子：
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作者：
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Avery, Leon
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作者：
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通讯作者：
KAPLAN, JM