Characterization of Epstein-Barr virus-encoded small RNA gene variations in virus associated lymphomas in Northern China
Characterization of Epstein-Barr virus-encoded small RNA gene variations in virus associated lymphomas in Northern China
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中国北方病毒相关淋巴瘤中 Epstein-Barr 病毒编码的小 RNA 基因变异特征
DOI：
10.1007/s00705-017-3278-4
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发表时间：
2017-02
影响因子：
2.7
通讯作者：
Luo Bing
中科院分区：
文献类型：
--
作者：
Zhao Zhenzhen;Sun Lingling;Liu Song;Shu Jun;Luo Bing
EB病毒（EBV）编码的小RNA（EBER 1和EBER 2）在EBV相关恶性肿瘤的所有形式的EBV潜伏期中高度表达。EBER基因变异及其与EB病毒相关疾病的关系仍然很难确定。为探讨EBER基因的变异模式及其在肿瘤发生中的作用，采用巢式PCR和DNA测序技术对中国北方非鼻咽癌（NPC）流行区101例恶性淋巴瘤的EBER基因序列进行了分析。此外，EBV 1型和2型分类是通过使用EBNA 2基因中类型特异性区域的巢式PCR测定进行的。EB-6 m是淋巴瘤的优势亚型（95.0%，96/101）。EBER亚型在四组淋巴瘤中的分布无显著性差异（p> 0.05），EBNA 2型在四组淋巴瘤中的分布也无显著性差异（p> 0.05）。EBER亚型在淋巴瘤中的分布与EBV相关胃癌（EBVaGC）和健康人咽拭子（TW）中的分布相似（p> 0.05），而与鼻咽癌的分布差异有统计学意义。淋巴瘤与其他三组EBNA 2型分布有显著性差异（p< 0.05）。淋巴瘤中1型和2型双重感染的比例高于GC、NPC和TW。101例淋巴瘤中11例（10.9%，11/101）发现7123 ntA → T突变，显著高于EBV相关胃癌（EBVaGC）（0%，0/50）和健康人咽洗液（TW）（3.3%，3/92）（p< 0.05）。这些结果表明，EBER亚型可能与淋巴瘤的发病无关，但7123 nt（A → T）点突变提供了一个新的研究领域。此外，有必要研究EBNA 2亚型混合感染在淋巴瘤形成中的作用。
Epstein-Barr virus (EBV)-encoded small RNAs (EBER1 and EBER2) are highly expressed in all forms of EBV latency in EBV-associated malignancies. EBER gene variations and their association with EBV-associated disease still remain poorly characterized. To investigate the patterns of EBER gene variations and their roles in tumorigenesis, EBER gene sequences were analyzed by nested-PCR and DNA sequencing in 101 lymphomas from Northern China, a non-nasopharyngeal carcinoma (NPC) endemic area. In addition, EBV type 1 and type 2 classifications were made by using nested-PCR assays across type-specific regions in the EBNA2 gene. EB-6m was the dominant subtype (95.0%, 96/101) in lymphoma. The distribution of the EBER subtypes in the four lymphoma groups was not significantly different (p> 0.05), neither was that of the EBNA2 type (p> 0.05). Compared with previous data in the same area, the distribution of EBER subtypes in lymphoma was similar to that in EBV-associated gastric carcinoma (EBVaGC) and throat washing (TW) from healthy donors (p> 0.05), but was significantly different from that of NPC. The EBNA2 type distribution between lymphoma and the other three groups was significantly different (p< 0.05). The proportion of type 1 and type 2 dual infections was higher in lymphoma than that in GC, NPC and TW. The mutation 7123nt A → T was identified in 11 of 101 (10.9%, 11/101) lymphomas, significantly more than that in EBV-associated gastric carcinomas (EBVaGC) (0%, 0/50) and throat washings (TWs) from healthy donors (3.3%, 3/92) (p< 0.05). These findings indicate that EBER subtypes may not be associated with pathogenesis of lymphoma, but that a point mutation at position 7123nt (A → T) provides a new area for further exploration. Furthermore it is necessary to investigate the role of EBNA2-subtype mixed infections in the establishment of lymphoma.
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影响因子：
5.2
作者：
Nishikawa J;Yoshiyama H;Iizasa H;Kanehiro Y;Nakamura M;Nishimura J;Saito M;Okamoto T;Sakai K;Suehiro Y;Yamasaki T;Oga A;Yanai H;Sakaida I
通讯作者：
Sakaida I
影响因子：
6.4
作者：
B. Borisch;Massimo Caioni;N. Hurwitz;C. Dommann-Scherrer;B. Odermatt;E. Waelti;R. Hubert Hubert-R.-Hubert-Hubert-1953014615;R. Kraft;J. Laissue
通讯作者：
B. Borisch;Massimo Caioni;N. Hurwitz;C. Dommann-Scherrer;B. Odermatt;E. Waelti;R. Hubert Hubert-R.-Hubert-Hubert-1953014615;R. Kraft;J. Laissue
影响因子：
3.4
作者：
KHAN, G;COATES, PJ;SLAVIN, G
通讯作者：
SLAVIN, G
影响因子：
5.4
作者：
Komano, J;Maruo, S;Takada, K
通讯作者：
Takada, K
DOI：
10.1038/npg.els.0002231
发表时间：
2001-04
期刊：
--
影响因子：
--
作者：
M. Rowe;A. Rickinson
通讯作者：
M. Rowe;A. Rickinson