A quantitative and dynamic model for plant stem cell regulation.
A quantitative and dynamic model for plant stem cell regulation.
复制标题
DOI：
10.1371/journal.pone.0003553
复制
发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Fleck C
中科院分区：
文献类型：
--
作者：
Geier F;Lohmann JU;Gerstung M;Maier AT;Timmer J;Fleck C
植物一生都维护着全能干细胞的池。这些干细胞嵌入了称为分生组织的专用组织中，这形成了生物体的生长点。参考植物拟南芥的芽顶分生组织被细分为几个不同的领域，这些结构域执行多种生物学功能，例如组织组织，细胞增殖和分化。生长和器官形成所需的细胞数量在植物寿命中变化，而分生组织的结构仍然非常恒定。因此，必须建立调节系统，这可以使芽顶分生组织内细胞增殖适应，同时将组织保持在组织水平。为了促进我们对这种动态组织行为的理解，我们在各种环境条件下测量了域的大小以及芽顶分生组织的细胞分裂速率，这会导致分生组织的适应性。基于我们的结果，我们开发了一个数学模型，以解释细胞池大小的依赖性调节细胞增殖和分化的变化，该调节能够正确预测CLV3和WUS过表达表型。尽管该模型在恒定生长条件下显示出干细胞稳态，但它预测了在变化条件下干细胞数的变化。与我们的实验数据一致，这种行为与细胞增殖的变化相关。因此，我们研究了不同的信号传导机制，尽管细胞增殖变化，但这些信号传导机制仍可以稳定干细胞数量。我们的结果将拟南芥在不同的环境条件和发育状态下的芽顶分生组织中的干细胞池维持的动态约束中阐明。
Plants maintain pools of totipotent stem cells throughout their entire life. These stem cells are embedded within specialized tissues called meristems, which form the growing points of the organism. The shoot apical meristem of the reference plant Arabidopsis thaliana is subdivided into several distinct domains, which execute diverse biological functions, such as tissue organization, cell-proliferation and differentiation. The number of cells required for growth and organ formation changes over the course of a plants life, while the structure of the meristem remains remarkably constant. Thus, regulatory systems must be in place, which allow for an adaptation of cell proliferation within the shoot apical meristem, while maintaining the organization at the tissue level. To advance our understanding of this dynamic tissue behavior, we measured domain sizes as well as cell division rates of the shoot apical meristem under various environmental conditions, which cause adaptations in meristem size. Based on our results we developed a mathematical model to explain the observed changes by a cell pool size dependent regulation of cell proliferation and differentiation, which is able to correctly predict CLV3 and WUS over-expression phenotypes. While the model shows stem cell homeostasis under constant growth conditions, it predicts a variation in stem cell number under changing conditions. Consistent with our experimental data this behavior is correlated with variations in cell proliferation. Therefore, we investigate different signaling mechanisms, which could stabilize stem cell number despite variations in cell proliferation. Our results shed light onto the dynamic constraints of stem cell pool maintenance in the shoot apical meristem of Arabidopsis in different environmental conditions and developmental states.
登录
查看更多内容
影响因子：
4.8
作者：
Menges, M;Hennig, L;Murray, JAH
通讯作者：
Murray, JAH
影响因子：
11.6
作者：
Laufs, P;Grandjean, O;Traas, J
通讯作者：
Traas, J
DOI：
10.1073/pnas.0509839103
发表时间：
2006-01-31
影响因子：
11.1
作者：
Jönsson, H;Heisler, MG;Mjolsness, E
通讯作者：
Mjolsness, E
影响因子：
7.4
作者：
Brand, U;Grünewald, M;Simon, R
通讯作者：
Simon, R
影响因子：
56.9
作者：
Reddy, GV;Meyerowitz, EM
通讯作者：
Meyerowitz, EM