Transcriptome-wide analysis to dissect the transcription factors orchestrating the phase change from vegetative to reproductive development in Larix kaempferi
Transcriptome-wide analysis to dissect the transcription factors orchestrating the phase change from vegetative to reproductive development in Larix kaempferi
复制标题
全转录组分析，剖析落叶松从营养发育到生殖发育阶段变化的转录因子
DOI：
10.1007/s11295-019-1376-z
复制
发表时间：
2019-08
影响因子：
2.4
通讯作者：
Li-Wang Qi
中科院分区：
文献类型：
--
作者：
Wei-Bo Xiang;Wan-Feng Li;Shou-Gong Zhang;Li-Wang Qi
林木衰老过程中从营养发育到生殖发育的阶段变化时间对于木材和种子生产非常重要。在之前的一项研究中，我们通过测量1年、2年、5年、10年、25年和50年落叶松最上部主茎的转录组变化，研究了老化对木材形成的影响。基于已发表的转录组数据，我们研究了幼年营养期（1 岁和 2 岁）和成年生殖期（25 岁和 50 岁）之间的转录组差异，以确定阶段变化背后的分子机制。总共有 12,789 个转录本被鉴定为差异表达基因，其中包括 573 个转录因子。进一步分析表明，属于8个家族的27个转录因子在老年和年轻生命阶段类别的所有四个比较中是共有的：（I）25岁与1岁； （二）50岁vs 1岁； （三）25岁 vs 2岁； (IV) 50 岁与 2 岁。对6个树龄组表达模式的分析表明，AP2和Dof家族成员在1年和2年生树中表达较高，在25年和50年树中表达较弱，而C3H、G2-like、GRAS、MYB相关和MADS家族成员则具有相反的模式。值得注意的是，MADS 家族的一种成员仅在 25 年和 50 年树龄的树木中被发现。这些结果表明，相变可能（1）发生在山奈生命的早期阶段，（2）受到不同转录因子的复杂调控网络的控制，其中一些转录因子已知在模型植物的相变中发挥作用。这些发现不仅为区分树木生长和发育的不同阶段提供了分子标记，也为遗传操作改善树木繁殖性状的潜在目标提供了分子标记，而且提高了我们对树木老化阶段变化的理解。
The timing of phase change from vegetative to reproductive development during aging of forest trees is important for wood and seed production. In a previous study, we investigated the effects of aging on wood formation by measuring the transcriptomic changes in the uppermost main stems of 1-, 2-, 5-, 10-, 25-, and 50-year-old Larix kaempferi. Based on the published transcriptome data, here we investigated the transcriptomic differences between the juvenile vegetative (1- and 2-year-old) and adult reproductive (25- and 50-year-old) phases to determine the molecular mechanisms underlying the phase change. In total, 12,789 transcripts were identified as differentially expressed genes, including 573 transcription factors. Further analysis showed that 27 transcription factors belonging to 8 families were common to all four comparisons between old and young life stage categories: (I) 25 vs 1 year old; (II) 50 vs 1 year old; (III) 25 vs 2 years old; and (IV) 50 vs 2 years old. The analysis of their expression patterns in six age categories showed that members of the AP2 and Dof families were expressed highly in 1- and 2-year-old trees, weakly in 25- and 50-year-old trees, while members of the C3H, G2-like, GRAS, MYB-related, and MADS families had the opposite patterns. Notably, one member of the MADS family was only detected in 25- and 50-year-old trees. These results suggest that the phase change might (1) occur in the early stage of the L. kaempferi lifetime and (2) be controlled by a complex regulatory network of different transcription factors, some of which are known to play roles in the phase change in model plants. These findings not only provide molecular markers to distinguish different stages of tree growth and development and potential targets for genetic manipulation to improve the reproductive traits of trees, but also improve our understanding of the phase change with aging in trees.
登录
查看更多内容
影响因子：
4.3
作者：
Huimin Xu-;Dechang Cao;Jinling Feng;Hongyan Wu;Jinxing Lin;Yanwei Wang
通讯作者：
Huimin Xu-;Dechang Cao;Jinling Feng;Hongyan Wu;Jinxing Lin;Yanwei Wang
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5.3
作者：
Curaba J;Talbot M;Li Z;Helliwell C
通讯作者：
Helliwell C
PlantTFDB 4.0：成为植物转录因子和调控相互作用的中心枢纽。
影响因子：
14.9
作者：
Jin J;Tian F;Yang DC;Meng YQ;Kong L;Luo J;Gao G
通讯作者：
Gao G
影响因子：
9.5
作者：
Z. Sung;Lingjing Chen;Y. Moon;K. Lertpiriyapong
通讯作者：
Z. Sung;Lingjing Chen;Y. Moon;K. Lertpiriyapong