Hydrostatic pressure independently increases elastin and collagen co-expression in small-diameter engineered arterial constructs.
Hydrostatic pressure independently increases elastin and collagen co-expression in small-diameter engineered arterial constructs.
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DOI：
10.1002/jbm.a.33019
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发表时间：
2011-03-15
影响因子：
4.9
通讯作者：
Wang, Yadong
中科院分区：
文献类型：
--
作者：
Crapo, Peter M.;Wang, Yadong
关键词：
先前的研究表明，体外静水压会增加平滑肌细胞 (SMC) 的增殖、迁移和细胞外基质的产生。我们通过在由聚癸二酸甘油酯 (PGS) 组成的管状、多孔、弹性支架上脉动灌注下培养原代成人动脉 SMC，设计了高度顺应性的小直径动脉结构。本研究调查了静水压对基于 PGS 的工程动脉结构的生物和机械特性的影响。在灌注过程中使用下游针阀升高压力，同时保持流速和脉动性，并通过压力直径测试和胶原蛋白和弹性蛋白的生化测定来评估结构。加压构建体含有的不溶性弹性蛋白是狒狒颈总动脉的一半，但顺应性明显较低，而在低静水压下培养的构建体含有的不溶性弹性蛋白是狒狒颈总动脉的三分之一，并且顺应性相似。静水压力显着增加了培养基中的构建体爆裂压力、胶原蛋白和不溶性弹性蛋白含量以及可溶性弹性蛋白浓度。所有动脉和结构在压力循环期间都表现出弹性恢复。静水压似乎不会影响 SMC、胶原蛋白 I 和 III 以及弹性蛋白的径向分布。这些结果为利用静水压控制工程平滑肌组织特性提供了见解。
Prior studies have demonstrated that smooth muscle cell (SMC) proliferation, migration, and extracellular matrix production increase with hydrostatic pressure in vitro. We have engineered highly compliant small-diameter arterial constructs by culturing primary adult arterial SMCs under pulsatile perfusion on tubular, porous, elastomeric scaffolds composed of poly(glycerol sebacate) (PGS). This study investigates the effect of hydrostatic pressure on the biological and mechanical properties of PGS-based engineered arterial constructs. Pressure was raised using a downstream needle valve during perfusion while preserving flow rate and pulsatility, and constructs were evaluated by pressure-diameter testing and biochemical assays for collagen and elastin. Pressurized constructs contained half as much insoluble elastin as baboon common carotid arteries but were significantly less compliant, while constructs cultured at low hydrostatic pressure contained one third as much insoluble elastin as baboon carotids and were similar in compliance. Hydrostatic pressure significantly increased construct burst pressure, collagen and insoluble elastin content, and soluble elastin concentration in culture medium. All arteries and constructs exhibited elastic recovery during pressure cycling. Hydrostatic pressure did not appear to affect radial distribution of SMCs, collagens I and III, and elastin. These results provide insights into the control of engineered smooth muscle tissue properties using hydrostatic pressure.
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影响因子：
4.9
作者：
Gao, Jin;Crapo, Peter;Wang, Yadong
通讯作者：
Wang, Yadong
影响因子：
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作者：
Crapo, Peter M.;Wang, Yadong
通讯作者：
Wang, Yadong