The BnGRF2 gene (GRF2-like gene from Brassica napus) enhances seed oil production through regulating cell number and plant photosynthesis.
The BnGRF2 gene (GRF2-like gene from Brassica napus) enhances seed oil production through regulating cell number and plant photosynthesis.
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DOI：
10.1093/jxb/ers066
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发表时间：
2012-06
影响因子：
6.9
通讯作者：
Wang HZ
中科院分区：
文献类型：
--
作者：
Liu J;Hua W;Yang HL;Zhan GM;Li RJ;Deng LB;Wang XF;Liu GH;Wang HZ
籽粒产量和含油量是油料作物育种中的两个重要的农业性状，许多功能基因的研究都集中在提高这两个性状上。本研究通过对高产油菜系（zy036和51070）的差异基因表达分析，在高产油菜系（zy036）中鉴定出了BnGRF2（甘蓝型生长调节因子2样基因）。为了阐明BnGRF2在油菜籽产油中的可能作用，分离了油菜GRF2基因的cDNA序列。Blastn结果显示，油菜中含有分别位于A基因组（A1和A3）和C基因组（C1和C6）的BnGRF2a/2b基因，并选择其显性表达基因BnGRF2a进行转基因研究。对35S-BnGRF2a转基因拟南芥的分析表明，过表达BnGRF2a可使种子油产量增加50%。此外，由于叶绿素含量的增加，BnGRF2a还诱导伸展叶片>增大20%，光合效率>提高40%。此外，转录组分析表明，与细胞增殖、光合作用和油脂合成相关的一些基因表达上调，这表明细胞数量和植物光合作用对种子重量和油脂含量的增加有贡献。由于35S-BnGRF2a转基因株系中雌蕊较长诱导自交受精效率较低，进一步利用Napin-BnGRF2a转基因株系对BnGRF2的功能进行了鉴定，结果表明，与野生型对照相比，BnGRF2的籽油产量也提高了约40%。结果表明，通过调控GRF2的表达水平可以改善油料作物的重要经济性状。
Seed yield and oil content are two important agricultural characteristics in oil crop breeding, and a lot of functional gene research is being concentrated on increasing these factors. In this study, by differential gene expression analyses between rapeseed lines (zy036 and 51070) which exhibit different levels of seed oil production, BnGRF2 (Brassica napus growth-regulating factor 2-like gene) was identified in the high oil-producing line zy036. To elucidate the possible roles of BnGRF2 in seed oil production, the cDNA sequences of the rapeseed GRF2 gene were isolated. The Blastn result showed that rapeseed contained BnGRF2a/2b which were located in the A genome (A1 and A3) and C genome (C1 and C6), respectively, and the dominantly expressed gene BnGRF2a was chosen for transgenic research. Analysis of 35S-BnGRF2a transgenic Arabidopsis showed that overexpressed BnGRF2a resulted in an increase in seed oil production of >50%. Moreover, BnGRF2a also induced a >20% enlargement in extended leaves and >40% improvement in photosynthetic efficiency because of an increase in the chlorophyll content. Furthermore, transcriptome analyses indicated that some genes associated with cell proliferation, photosynthesis, and oil synthesis were up-regulated, which revealed that cell number and plant photosynthesis contributed to the increased seed weight and oil content. Because of less efficient self-fertilization induced by the longer pistil in the 35S-BnGRF2a transgenic line, Napin-BnGRF2a transgenic lines were further used to identify the function of BnGRF2, and the results showed that seed oil production also could increase >40% compared with the wild-type control. The results suggest that improvement to economically important characteristics in oil crops may be achieved by manipulation of the GRF2 expression level.
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影响因子：
4.9
作者：
Kozuka, T;Horiguchi, G;Tsukaya, H
通讯作者：
Tsukaya, H
影响因子：
13.8
作者：
Graef, George;LaVallee, Bradley J.;Clemente, Tom E.
通讯作者：
Clemente, Tom E.
影响因子：
7.2
作者：
Kim, JH;Choi, DS;Kende, H
通讯作者：
Kende, H
甘蓝型油菜种子含油量的母体控制：长角果壁光合作用的作用
影响因子：
7.2
作者：
Hua, Wei;Li, Rong-Jun;Wang, Han-Zhong
通讯作者：
Wang, Han-Zhong
影响因子：
7.4
作者：
Beisson, F;Koo, AJK;Ohlrogge, JB
通讯作者：
Ohlrogge, JB