Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.
Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.
复制标题
强直性脊柱炎和类风湿关节炎患者外周血中 Th22 细胞和 Th17 细胞的频率增加
DOI：
10.1371/journal.pone.0031000
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li JM
中科院分区：
文献类型：
--
作者：
Zhang L;Li YG;Li YH;Qi L;Liu XG;Yuan CZ;Hu NW;Ma DX;Li ZF;Yang Q;Li W;Li JM
研究背景辅助性T细胞（Th）22参与炎症性疾病的发病机制。Th 22细胞在强直性脊柱炎（AS）和类风湿性关节炎（RA）的病理生理中的作用尚不清楚。因此，我们检测了AS患者和RA患者外周血（PB）中Th 22细胞、Th 17细胞和Th 1细胞的频率，并与健康对照以及骨关节炎患者进行比较。研究设计与方法：32例AS患者、20例RA患者、10例OA患者和20例健康对照者。采用流式细胞术检测AS、RA、OA患者及健康对照者外周血IL-22、IL-17和IFN-γ的表达。采用酶联免疫吸附法检测血浆IL-22和IL-17水平。结果AS和RA患者Th 22细胞、Th 17细胞和IL-22水平明显高于OA患者和健康对照组。AS和RA患者Th 22细胞与Th 17细胞、IL-22呈正相关。而IL-22与Th 17细胞的表达仅在AS患者中呈正相关，而在RA患者中无相关性。此外，Th 22细胞和Th 17细胞的百分比仅在RA患者中与疾病活动性呈正相关，而在AS患者中不相关。结论AS和RA患者外周血中Th 22和Th 17细胞均升高。提示Th 22细胞和Th 17细胞可能参与了AS和RA的发病机制，Th 22细胞和Th 17细胞可能是治疗干预的合理细胞靶点。
Background T-helper (Th) 22 is involved in the pathogenesis of inflammatory diseases. The roles of Th22 cells in the pathophysiological of ankylosing spondylitis (AS) and rheumatoid arthritis (RA) remain unsettled. So we examined the frequencies of Th22 cells, Th17 cells and Th1 cells in peripheral blood (PB) from patients with AS and patients with RA compared with both healthy controls as well as patients with osteoarthritis. Design and Methods We studied 32 AS patients, 20 RA patients, 10 OA patients and 20 healthy controls. The expression of IL-22, IL-17 and IFN-γ were examined in AS, RA, OA patients and healthy controls by flow cytometry. Plasma IL-22 and IL-17 levels were examined by enzyme-linked immunosorbent assay. Results Th22 cells, Th17 cells and interleukin-22 were significantly elevated in AS and RA patients compared with OA patients and healthy controls. Moreover, Th22 cells showed positive correlation with Th17 cells as well as interleukin-22 in AS and RA patients. However, positive correlation between IL-22 and Th17 cells was only found in AS patients not in RA patients. In addition, the percentages of both Th22 cells and Th17 cells correlated positively with disease activity only in RA patients not in AS patients. Conclusions The frequencies of both Th22 cells and Th17 cells were elevated in PB from patients with AS and patients with RA. These findings suggest that Th22 cells and Th17 cells may be implicated in the pathogenesis of AS and RA, and Th22 cells and Th17 cells may be reasonable cellular targets for therapeutic intervention.
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影响因子：
27.4
作者：
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