mRNA and miRNA Profiles of Exosomes from Cultured Tumor Cells Reveal Biomarkers Specific for HPV16-Positive and HPV16-Negative Head and Neck Cancer.
mRNA and miRNA Profiles of Exosomes from Cultured Tumor Cells Reveal Biomarkers Specific for HPV16-Positive and HPV16-Negative Head and Neck Cancer.
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DOI：
10.3390/ijms21228570
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发表时间：
2020-11-13
影响因子：
5.6
通讯作者：
Whiteside TL
中科院分区：
文献类型：
--
作者：
Ludwig S;Sharma P;Wise P;Sposto R;Hollingshead D;Lamb J;Lang S;Fabbri M;Whiteside TL
人乳头瘤病毒（HPV）（+）和HPV（−）头颈癌（HNC）细胞与宿主免疫系统的相互作用知之甚少。最近，我们确定了HPV（+）与HPV（-）细胞产生的外泌体的分子和功能差异，表明外泌体的遗传货物可能在HPV相关的HNC中识别新的生物标志物。通过尺寸排阻色谱法从三种HPV（+）和两种HPV（-）HNC细胞系的上清液中分离外泌体。通过qRT-PCR分析配对的细胞裂解物和外来体的信使RNA（mRNA），并通过nanostring分析分析微小RNA（miR）含量。HPV（+）与HPV（-）细胞的mRNA谱不同，HPV（+）细胞中EGFR、TP 53和HSPA 1 A/B过表达，HPV（-）细胞中IL 6、FAS和DPP 4过表达。HPV（+）或HPV（−）外泌体的mRNA谱与其亲本细胞的货物相似。细胞裂解物中的miR表达谱鉴定了HPV（-）细胞中表达的8个miR，而HPV（+）细胞中表达的14个miR。miR-205- 5 p仅在HPV（+）外泌体中表达，而miR-1972仅在HPV（-）外泌体中检测到。我们发现HPV（+）和HPV（−）外泌体重现了其亲本细胞的mRNA表达谱。miR的表达取决于HPV状态，HPV（+）中的miR-205- 5 p和HPV（-）外泌体中的miR-1972成为潜在的区分HPV相关生物标志物。
Human papillomavirus (HPV)(+) and HPV(−) head and neck cancer (HNC) cells’ interactions with the host immune system are poorly understood. Recently, we identified molecular and functional differences in exosomes produced by HPV(+) vs. HPV(−) cells, suggesting that genetic cargos of exosomes might identify novel biomarkers in HPV-related HNCs. Exosomes were isolated by size exclusion chromatography from supernatants of three HPV(+) and two HPV(−) HNC cell lines. Paired cell lysates and exosomes were analyzed for messenger RNA (mRNA) by qRT-PCR and microRNA (miR) contents by nanostring analysis. The mRNA profiles of HPV(+) vs. HPV(−) cells were distinct, with EGFR, TP53 and HSPA1A/B overexpressed in HPV(+) cells and IL6, FAS and DPP4 in HPV(−) cells. The mRNA profiles of HPV(+) or HPV(−) exosomes resembled the cargo of their parent cells. miR expression profiles in cell lysates identified 8 miRs expressed in HPV(−) cells vs. 14 miRs in HPV(+) cells. miR-205-5p was exclusively expressed in HPV(+) exosomes, and miR-1972 was only detected in HPV(−) exosomes. We showed that HPV(+) and HPV(−) exosomes recapitulated the mRNA expression profiles of their parent cells. Expression of miRs was dependent on the HPV status, and miR-205-5p in HPV(+) and miR-1972 in HPV(−) exosomes emerge as potential discriminating HPV-associated biomarkers.
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影响因子：
--
作者：
Ding G;Zhou L;Qian Y;Fu M;Chen J;Chen J;Xiang J;Wu Z;Jiang G;Cao L
通讯作者：
Cao L
影响因子：
4.2
作者：
Chiba M;Kimura M;Asari S
通讯作者：
Asari S
DOI：
10.1002/hed.21475
发表时间：
2011-04
影响因子：
2.9
作者：
Wald, Abigail I.;Hoskins, Elizabeth E.;Wells, Susanne I.;Ferris, Robert L.;Khan, Saleem A.
通讯作者：
Khan, Saleem A.
DOI：
10.1007/978-3-319-43580-0_2
发表时间：
2017-01-01
期刊：
HPV INFECTION IN HEAD AND NECK CANCER
影响因子：
--
作者：
Castellsague, Xavier;Mena, Marisa;Alemany, Laia
通讯作者：
Alemany, Laia
影响因子：
4.6
作者：
Muller L;Mitsuhashi M;Simms P;Gooding WE;Whiteside TL
通讯作者：
Whiteside TL