Bacterial microbiota profiling in gastritis without Helicobacter pylori infection or non-steroidal anti-inflammatory drug use.
Bacterial microbiota profiling in gastritis without Helicobacter pylori infection or non-steroidal anti-inflammatory drug use.
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DOI：
10.1371/journal.pone.0007985
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发表时间：
2009-11-24
期刊：
影响因子：
3.7
通讯作者：
Ding C
中科院分区：
文献类型：
--
作者：
Li XX;Wong GL;To KF;Wong VW;Lai LH;Chow DK;Lau JY;Sung JJ;Ding C
最近胃粘膜16S核糖体RNA基因（rRNA）分子谱揭示了微生物群的惊人复杂性。幽门螺杆菌感染和非甾体抗炎药（NSAID）的使用是胃炎和消化性溃疡的两个主要原因。然而，人们对胃微生物群的其他成员与胃疾病之间的关系知之甚少。本研究通过对16S rRNA的克隆和测序，分析了正常胃炎患者和胃炎患者的胃微生物群。从8个细菌门中鉴定出133个种型。发现胃微生物群与粘膜紧密粘附。从活检中成功培养了11种链球菌。在任何鉴定的门内，一到两个属代表了大多数克隆。我们进一步开发了两种实时定量PCR方法来量化厚壁菌门和链球菌属的相对丰度。胃窦性胃炎患者中厚壁菌门和厚壁菌门内链球菌属的丰度明显高于正常对照组。这一研究表明，属的分类单元水平在很大程度上可以代表更高的分类单元，如门。胃炎中微生物群组成改变的临床相关性和机制需要进一步的功能研究。
Recent 16S ribosomal RNA gene (rRNA) molecular profiling of the stomach mucosa revealed a surprising complexity of microbiota. Helicobacter pylori infection and non-steroidal anti-inflammatory drug (NSAID) use are two main contributors to gastritis and peptic ulcer. However, little is known about the association between other members of the stomach microbiota and gastric diseases. In this study, cloning and sequencing of the 16S rRNA was used to profile the stomach microbiota from normal and gastritis patients. One hundred and thirty three phylotypes from eight bacterial phyla were identified. The stomach microbiota was found to be closely adhered to the mucosa. Eleven Streptococcus phylotypes were successfully cultivated from the biopsies. One to two genera represented a majority of clones within any of the identified phyla. We further developed two real-time quantitative PCR assays to quantify the relative abundance of the Firmicutes phylum and the Streptococcus genus. Significantly higher abundance of the Firmicutes phylum and the Streptococcus genus within the Firmicutes phylum was observed in patients with antral gastritis, compared with normal controls. This study suggests that the genus taxon level can largely represent much higher taxa such as the phylum. The clinical relevance and the mechanism underlying the altered microbiota composition in gastritis require further functional studies.
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影响因子：
3
作者：
Monstein, HJ;Tiveljung, A;Jonasson, J
通讯作者：
Jonasson, J
影响因子：
5.8
作者：
Huber, T;Faulkner, G;Hugenholtz, P
通讯作者：
Hugenholtz, P
影响因子：
4.5
作者：
Hawkins, NJ;Bariol, C;Ward, RL
通讯作者：
Ward, RL
DOI：
10.1073/pnas.0308766101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Lepp, PW;Brinig, MM;Relman, DA
通讯作者：
Relman, DA
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI