Inositol 1,4,5-trisphosphate-gated conductance in isolated rat olfactory neurons.
Inositol 1,4,5-trisphosphate-gated conductance in isolated rat olfactory neurons.
复制标题
离体大鼠嗅觉神经元中肌醇 1,4,5-三磷酸门控电导。
DOI：
10.1152/jn.1994.71.2.595
复制
发表时间：
1994
影响因子：
2.5
通讯作者：
Restrepo,D
中科院分区：
文献类型：
--
作者：
Okada,Y;Teeter,JH;Restrepo,D
1.在全细胞膜片钳技术下，分析了从膜片吸管细胞内应用1，4，5-三磷酸肌醇（IP 3）对分离的大鼠嗅觉神经元的影响。2.在K（+）-内部溶液中的10 μ M 1，4，5-IP 3的细胞内透析诱导35.8 +/- 10.5（SD）mV的持续去极化（n = 16）。在用IP 3透析的75%的细胞中观察到IP 3诱导的反应，但当10 μ M钌红也包括在移液管溶液中时（4个细胞）没有观察到IP 3诱导的反应。较低浓度（50-100 nM）的2，4，5-IP 3在8个嗅觉神经元中的5个中诱导了与1，4，5-IP 3产生的反应相似的反应。3. IP 3门控电流与K（+）内溶液的稳态I-V关系可分为两种类型：外向整流型和N型。在Cs（+）-内溶液中观察到向外整流和线性模式。4. IP 3诱导的电流被抑制由外部Cd 2+（1 mM）。在K（+）-内溶液中，外向整流型和N型Cd（2+）-可逆电流的翻转电位分别为-16.1mV（n = 2）和-29.0 +/-7.1mV（n = 3）。在Cs（+）-内溶液中的翻转电位为-5.9 +/-6.8mV（n = 5）。6.相反，Ca（2+）-离子载体，离子霉素（5 μ M）超极化的嗅觉神经元，大大增强了外向电流在正保持膜电位。7.结果表明，IP 3对大鼠嗅神经元的去兴奋作用不受细胞内Ca ~（2+）的介导。
1. The effect of intracellular application of inositol 1,4,5-trisphosphate (IP3) from the patch pipette was analyzed in isolated rat olfactory neurons under whole-cell patch clamp. 2. Intracellular dialysis of 10 microM 1,4,5-IP3 in K(+)-internal solution induced a sustained depolarization of 35.8 +/- 10.5 (SD) mV (n = 16). The IP3-induced response was observed in 75% of the cells dialyzed with IP3 but not when 10 microM ruthenium red was also included in the pipette solution (4 cells). Lower concentrations (50-100 nM) of 2,4,5-IP3 induced similar responses to those produced by 1,4,5-IP3 in five of eight olfactory neurons. 3. Steady-state I-V relationships of IP3-gated currents with K(+)-internal solution were classified into two types: outwardly rectifying and N-shaped. In Cs(+)-internal solution outwardly rectifying and linear patterns were observed. 4. The IP3-induced currents were inhibited by external Cd2+ (1 mM). The reversal potentials of the Cd(2+)-inhibitable currents were -16.1 mV (n = 2) and -29.0 +/- 7.1 mV (n = 3) for the outwardly rectifying and N-shaped types, respectively, in K(+)-internal solution. The reversal potential was -5.9 +/- 6.8 mV (n = 5) in the Cs(+)-internal solution. 6. In contrast, the Ca(2+)-ionophore, ionomycin (5 microM) hyperpolarized the olfactory neurons and greatly potentiated the outward currents at positive holding membrane potential. 7. The data suggest that IP3 can depolarize rat olfactory neurons without mediation by intracellular Ca2+.
登录
查看更多内容
Takashi Kurahashi：“蝾螈的孤立嗅觉受体细胞中的 Ca^<2+> 依赖性适应性特性。” 515. 261-268 (1990)。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--
分离的大鼠嗅觉纤毛中存在 InsP3 门控通道蛋白的证据。
DOI：
10.1152/ajpcell.1992.263.3.c667
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Restrepo,D;Teeter,JH;Honda,E;Boyle,AG;Marecek,JF;Prestwich,GD;Kalinoski,DL
通讯作者：
Kalinoski,DL
影响因子：
3.5
作者：
BREER, H;BOEKHOFF, I
通讯作者：
BOEKHOFF, I
影响因子：
3.8
作者：
Frings, S;Lynch, J W;Lindemann, B
通讯作者：
Lindemann, B
影响因子：
56.9
作者：
RESTREPO, D;MIYAMOTO, T;TEETER, JH
通讯作者：
TEETER, JH