Intervertebral disc cell response to dynamic compression is age and frequency dependent.
Intervertebral disc cell response to dynamic compression is age and frequency dependent.
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DOI：
10.1002/jor.20814
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发表时间：
2009-06
影响因子：
2.8
通讯作者：
Iatridis, James C.
中科院分区：
文献类型：
--
作者：
Korecki, Casey L.;Kuo, Catherine K.;Tuan, Rocky S.;Iatridis, James C.
椎间盘细胞外基质的维持受机械负荷、营养、基质蛋白和细胞因子积累的调节，而这些均受衰老和退变的影响。有证据表明，细胞老化可能导致细胞外基质产生的数量和质量的改变。本研究的目的是研究负载和成熟（衰老的一个子集）的作用，以及这两个因素在受控三维培养环境下椎间盘细胞基因表达和生物合成中的相互作用。从幼龄（4-6个月）和成熟期（18-24个月）牛尾纤维环和髓核组织中分离细胞。将分离的细胞接种到海藻酸盐中，在0.1、1或3 Hz的频率下动态压缩7天，或作为自由肿胀对照组。7 d后，检测DNA和硫代糖胺聚糖含量，实时定量逆转录-聚合酶链反应检测I型和II型胶原蛋白、聚集蛋白和基质金属蛋白酶-3基因表达。结果表明，成熟在椎间盘稳态中起重要作用，并影响细胞对机械负荷的反应。虽然在三维培养中分离的椎间盘细胞对机械压缩有反应，但加载频率的影响很小。细胞表型和生物合成速率的改变似乎是细胞成熟过程的一个属性，可能独立于与营养损失和椎间盘退变相关的细胞微环境的变化。与年轻细胞相比，成熟细胞在机械负荷下产生或保留细胞外基质成分的能力可能下降。
The maintenance of the intervertebral disc extracellular matrix is regulated by mechanical loading, nutrition, and the accumulation of matrix proteins and cytokines that are affected by both aging and degeneration. Evidence suggests that cellular aging may lead to alterations in the quantity and quality of extracellular matrix produced. The aims of this study were to examine the role of loading and maturation (a subset of aging), and the interaction between these two factors in intervertebral disc cell gene expression and biosynthesis in a controlled 3D culture environment. Cells were isolated from young (4–6 months) and mature (18–24 months) bovine caudal annulus fibrosus and nucleus pulposus tissue. Isolated cells were seeded into alginate and dynamically compressed for 7 days at either 0.1, 1, or 3 Hz or maintained as a free-swelling control. After 7 days, DNA and sulfated glycosaminoglycan contents were analyzed along with real time, quantitative reverse transcription-polymerase chain reaction analysis for collagen types I and II, aggrecan, and matrix metalloproteinase-3 gene expression. Results suggest that maturation plays an important role in intervertebral disc homeostasis and influences the cell response to mechanical loading. While isolated intervertebral disc cells responded to mechanical compression in 3D culture, the effect of loading frequency was minimal. Altered cellular phenotype and biosynthesis rates appear to be an attribute of the cell maturation process, potentially independent of changes in cellular microenvironment associated with lost nutrition and disc degeneration. Mature cells may have a decreased capacity to create or retain extracellular matrix components in response to mechanical loading compared to young cells.
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影响因子：
6.9
作者：
Chen, J;Yan, W;Setton, LA
通讯作者：
Setton, LA
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