Altered maternal profiles in corticotropin-releasing factor receptor 1 deficient mice.
Altered maternal profiles in corticotropin-releasing factor receptor 1 deficient mice.
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皮质激素释放因子受体1缺陷小鼠的母体谱改变。
DOI：
10.1186/1471-2202-8-17
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发表时间：
2007-03-01
期刊：
影响因子：
2.4
通讯作者：
Stevenson, Sharon A.
中科院分区：
文献类型：
--
作者：
Gammie, Stephen C.;Bethea, Emily D.;Stevenson, Sharon A.
在哺乳期间，中枢神经系统对致焦虑的神经肽、促肾上腺皮质激素释放因子 (CRF) 的反应较弱。此外，中央注射 CRF 可以抑制母体攻击性和一些母体行为，表明哺乳期间 CRF 神经传递的减少支持母体行为。在这项研究中，我们检查了缺失 CRF 受体 1 (CRFR1) 的小鼠的母体概况。基因敲除 (CRFR1-/-) 小鼠的后代是杂合子，以抵消低母体糖皮质激素对幼仔存活可能产生的有害影响，并且所有小鼠均含有混合的 50:50 近交/远交背景，以改善整体母体概况和繁殖力。相对于同窝野生型 (WT) 对照，CRFR1-/- 小鼠在每个测试日的总护理时间上表现出显着的缺陷，包括高弓背。与减少哺乳相一致的是，CRFR1 缺陷母鼠的幼崽体重明显低于 WT 后代。 WT 小鼠在产后第 2 天以及对两个测试日进行平均时，幼崽的舔舐和梳理行为显着较高，但在第 3 天则不然。 CRFR1-/- 小鼠在第 2 天的离巢时间较长，但在第 3 天或对测试日进行平均时则不然。当将这一指标作为在巢上的时间的比例进行检查时，幼崽的舔舐和梳理行为在第二天没有差异。 CRFR1-/- 小鼠在第 3 天和测试平均时表现出明显更高的筑巢能力。各组之间的平均幼仔数量几乎相同，并且没有发生幼仔死亡。 CRFR1-/- 小鼠的母亲攻击性始终较低，在某些测量中这些差异接近，但未达到显着性。由于差异较大，一般攻击结果被视为初步结果。就对入侵者的攻击部位而言，CRFR1-/-小鼠在第5天以及对测试进行平均时表现出对入侵者腹部的攻击明显较少。不同基因型在高架十字迷宫中的表现相似。不同基因型之间内侧视前核中的 Egr-1 表达差异和终纹床核中的 c-Fos 表达差异表明基因丢失可能会改变行为输出。总而言之，结果表明完整 CRFR1 受体的存在支持养育行为的某些方面。
During lactation, the CNS is less responsive to the anxiogenic neuropeptide, corticotropin-releasing factor (CRF). Further, central injections of CRF inhibit maternal aggression and some maternal behaviors, suggesting decreased CRF neurotransmission during lactation supports maternal behaviors. In this study, we examined the maternal profile of mice missing the CRF receptor 1 (CRFR1). Offspring of knockout (CRFR1-/-) mice were heterozygote to offset possible deleterious effects of low maternal glucocorticoids on pup survival and all mice contained a mixed 50:50 inbred/outbred background to improve overall maternal profiles and fecundity. Relative to littermate wild-type (WT) controls, CRFR1-/- mice exhibited significant deficits in total time nursing, including high arched-back, on each test day. Consistent with decreased nursing, pups of CRFR1-deficient dams weighed significantly less than WT offspring. Licking and grooming of pups was significantly higher in WT mice on postpartum Day 2 and when both test days were averaged, but not on Day 3. Time off nest was higher for CRFR1-/- mice on Day 2, but not on Day 3 or when test days were averaged. Licking and grooming of pups did not differ on Day 2 when this measure was examined as a proportion of time on nest. CRFR1-/- mice showed significantly higher nest building on Day 3 and when tests were averaged. Mean pup number was almost identical between groups and no pup mortality occurred. Maternal aggression was consistently lower in CRFR1-/- mice and in some measures these differences approached, but did not reach significance. Because of high variance, general aggression results are viewed as preliminary. In terms of sites of attacks on intruders, CRFR1-/- mice exhibited significantly fewer attacks to the belly of the intruder on Day 5 and when tests were averaged. Performance on the elevated plus maze was similar between genotypes. Egr-1 expression differences in medial preoptic nucleus and c-Fos expression differences in bed nucleus of stria terminalis between genotype suggest possible sites where loss of gene alters behavioral output. Taken together, the results suggest that the presence of an intact CRFR1 receptor supports some aspects of nurturing behavior.
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影响因子：
2.7
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通讯作者：
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