Successful weight regain attenuation by autologous fecal microbiota transplantation is associated with non-core gut microbiota changes during weight loss; randomized controlled trial.
Successful weight regain attenuation by autologous fecal microbiota transplantation is associated with non-core gut microbiota changes during weight loss; randomized controlled trial.
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DOI：
10.1080/19490976.2023.2264457
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发表时间：
2023-12
期刊：
影响因子：
12.2
通讯作者：
中科院分区：
文献类型：
--
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我们以前报道过，自体粪便微生物移植（aFMT），6个月的生活方式干预后，衰减随后的体重反弹和胰岛素反弹的参与者消耗高多酚绿色地中海饮食。在这里，我们探讨了在减肥阶段（0-6个月）核心（丰富）与非核心（低丰度）肠道微生物组分类群组分的特定变化是否与aFMT后的体重维持有差异。82名腹部肥胖/血脂异常的参与者（年龄= 52岁; 6个月体重减轻=-8.3 kg）提供粪便样本（0个月，6个月）。将冷冻的6个月的粪便样品加工成1 g不透明且无味的aFMT胶囊。参与者被随机分配接受100粒含有自己粪便微生物群或安慰剂的胶囊，在8 m-14 m的10次给药中（依从率> 90%）。使用鸟枪法宏基因组测序评估肠道微生物组组成。非核心分类群定义为受试者的患病率≤ 66%。总共分析了450个物种。基线时，13.3%被归类为核心，厚壁菌门的核心比例最高。在6 m失重阶段，非核心物种的丰度变化大于核心物种（P < .0001）。核心和非核心分类群组分的受试者特异性变化具有强相关性（Jaccard指数; r = 0.54; P <0.001）。在aFMT治疗后，只有核心分类群变化低6 m和非核心分类群变化高的参与者避免了8-14 m的体重恢复（aFMT =-0.58 ± 2.4 kg，相应的安慰剂组= 3.18 ± 3.5 kg; P = 0.02）。在线性回归模型中，低核心/高非核心6 m变化是与8-14 m体重恢复减弱显著相关的唯一组合（P = 0.038;分类群模式/治疗干预相互作用P = 0.002）。在减肥过程中，非核心、低丰度分类群的高度变化可能介导aFMT治疗成功维持减肥。ClinicalTrials.gov：NCT03020186
We previously reported that autologous-fecal-microbiota-transplantation (aFMT), following 6 m of lifestyle intervention, attenuated subsequent weight regain and insulin rebound for participants consuming a high-polyphenol green-Mediterranean diet. Here, we explored whether specific changes in the core (abundant) vs. non-core (low-abundance) gut microbiome taxa fractions during the weight-loss phase (0–6 m) were differentially associated with weight maintenance following aFMT. Eighty-two abdominally obese/dyslipidemic participants (age = 52 years; 6 m weightloss = −8.3 kg) who provided fecal samples (0 m, 6 m) were included. Frozen 6 m’s fecal samples were processed into 1 g, opaque and odorless aFMT capsules. Participants were randomly assigned to receive 100 capsules containing their own fecal microbiota or placebo over 8 m-14 m in ten administrations (adherence rate > 90%). Gut microbiome composition was evaluated using shotgun metagenomic sequencing. Non-core taxa were defined as ≤ 66% prevalence across participants. Overall, 450 species were analyzed. At baseline, 13.3% were classified as core, and Firmicutes presented the highest core proportion by phylum. During 6 m weight-loss phase, abundance of non-core species changed more than core species (P < .0001). Subject-specific changes in core and non-core taxa fractions were strongly correlated (Jaccard Index; r = 0.54; P < .001). Following aFMT treatment, only participants with a low 6 m change in core taxa, and a high change in non-core taxa, avoided 8–14 m weight regain (aFMT = −0.58 ± 2.4 kg, corresponding placebo group = 3.18 ± 3.5 kg; P = .02). In a linear regression model, low core/high non-core 6 m change was the only combination that was significantly associated with attenuated 8–14 m weight regain (P = .038; P = .002 for taxa patterns/treatment intervention interaction). High change in non-core, low-abundance taxa during weight-loss might mediate aFMT treatment success for weight loss maintenance. ClinicalTrials.gov: NCT03020186
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影响因子：
64.8
作者：
Chassaing B;Koren O;Goodrich JK;Poole AC;Srinivasan S;Ley RE;Gewirtz AT
通讯作者：
Gewirtz AT
影响因子：
12.3
作者：
Rinott E;Meir AY;Tsaban G;Zelicha H;Kaplan A;Knights D;Tuohy K;Scholz MU;Koren O;Stampfer MJ;Wang DD;Shai I;Youngster I
通讯作者：
Youngster I
影响因子：
12.3
作者：
Lloyd-Price J;Abu-Ali G;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
29.4
作者：
Jie, Zhuye;Yu, Xinlei;Cai, Kaiye
通讯作者：
Cai, Kaiye
DOI：
10.1073/pnas.2104429118
发表时间：
2021-12-21
影响因子：
11.1
作者：
Neu, Alexander T.;Allen, Eric E.;Roy, Kaustuv
通讯作者：
Roy, Kaustuv