Deep Tissue Imaging with Multiphoton Fluorescence Microscopy.
Deep Tissue Imaging with Multiphoton Fluorescence Microscopy.
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DOI:
10.1016/j.cobme.2017.09.004
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发表时间:
2017-12
影响因子:
3.9
通讯作者:
Dunn AK
中科院分区:
文献类型:
--
作者:
Miller DR;Jarrett JW;Hassan AM;Dunn AK
We present a review of imaging deep-tissue structures with multiphoton microscopy. We examine the effects of light scattering and absorption due to the optical properties of biological sample and identify 1,300 nm and 1,700 nm as ideal excitation wavelengths. We summarize the availability of fluorophores for multiphoton microscopy as well as ultrafast laser sources to excite available fluorophores. Lastly, we discuss the applications of multiphoton microscopy for neuroscience.
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