Two-photon optical properties of near-infrared dyes at 1.55 μm excitation.

Two-photon optical properties of near-infrared dyes at 1.55 μm excitation.
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DOI:
10.1021/jp207618e
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发表时间:
2011-10-06
影响因子:
3.3
通讯作者:
Achilefu, Samuel
Achilefu, Samuel
中科院分区:
化学3区
文献类型:
--
作者:
Berezin, Mikhail Y.;Zhan, Chun;Lee, Hyeran;Joo, Chulmin;Akers, Walter J.;Yazdanfar, Siavash;Achilefu, Samuel

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使用1.55μm兴奋的2P荧光分光光度计评估了两频(2p)的光学特性染料DTTC显示出最高的2p作用横截面(〜103±19 gm)和相对较高的2P激发荧光寿命,可用作2P分子成像问题的脚手架。生命周期也对溶液极性高度敏感,为其在光学成像中的使用和总体探测环境因素的机制提供了其他其他参数,这项研究证明了NIR染料2p性质的定量测量,并确定了设计分子的基础NIR地区2p成像应用的问题。
Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross-sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC showed the highest 2P action cross-section (~103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.
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