Genetic diversity of Toxoplasma gondii isolates from chickens from Brazil.
Genetic diversity of Toxoplasma gondii isolates from chickens from Brazil.
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DOI：
10.1016/j.vetpar.2008.07.036
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发表时间：
2008-11-07
影响因子：
2.6
通讯作者：
Su, C.
中科院分区：
文献类型：
--
作者：
Dubey, J. P.;Velmurugan, G. V.;Chockalingam, A.;Pena, H. F. J.;de Oliveira, L. Nunes;Leifer, C. A.;Gennari, S. M.;Oliveira, L. M. G. Bahia;Su, C.
直到最近，弓形虫被认为是克隆的，遗传变异性很小。最近的研究表明T.来自巴西的弓形虫分离株在遗传和生物学上与弓形虫不同。美国和欧洲的弓形虫分离株。在本研究中，我们重新分型151自由放养的鸡从巴西分离，包括117个新分离的样品从11个地理区域（阿拉戈斯，巴伊亚，塞阿拉，马拉尼昂，巴拉那，伯南布哥，里约热内卢，北格兰德河，圣保罗，塞尔吉佩，和Rondonia）和34个以前报道的分离株从最北部（帕拉）和最南部（南里奥格兰德）。采用SAG 1、SAG 2、SAG 3、BTUB、GRA 6、c22-8、c29-2、L358、PK 1和Apico等10个PCR-RFLP标记对所有分离株进行基因分型。对151株T.弓形虫分离株有58种基因型。这些基因型中有一半（29/58）具有单一分离物，另一半基因型具有两个或两个以上分离物的特征。151株分离株中只有1株为I型克隆株，5株为III型克隆株。2株分离株为混合感染。不存在克隆II型菌株。一个菌株是II型在所有位点，除了BTUB。结果表明，中国南方红豆杉具有较高的遗传多样性。从巴西分离的弓形虫。
Until recently, Toxoplasma gondii was considered clonal with very little genetic variability. Recent studies indicate that T. gondii isolates from Brazil are genetically and biologically different from T. gondii isolates from USA and Europe. In the present study, we retyped 151 free range chicken isolates from Brazil including 117 newly isolated samples from 11 geographically areas (Alagoas, Bahia, Ceará, Maranhão, Paraná, Pernambuco, Rio de Janeiro, Rio Grande do Norte, São Paulo, Sergipe, and Rondonia) and 34 previously reported isolates from the very north (Pará) and the very south (Rio Grande do Sul). Ten PCR-RFLP markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico were used to genotype all isolates. Overall analysis of 151 T. gondii isolates revealed 58 genotypes. Half (29/58) of these genotypes had single isolate and the other half of the genotypes were characterized with two or more isolates. Only 1 of 151 isolates was clonal Type I strain and 5 were clonal Type III strains. Two isolates had mixed infections. Clonal Type II strain was absent. One strain was Type II at all loci, except BTUB. The results confirm high genetic diversity of T. gondii isolates from Brazil.
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影响因子：
6.4
作者：
Ajzenberg, D;Cogné, N;Dardé, ML
通讯作者：
Dardé, ML
影响因子：
2.6
作者：
Dubey, J. P.;Sundar, N.;Su, C.
通讯作者：
Su, C.
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通讯作者：
Sibley, LD
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作者：
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通讯作者：
Lehmann, T
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4
作者：
Ajzenberg, D;Bañuls, AL;Dardé, ML
通讯作者：
Dardé, ML