DNA-binding specificity of rice mariner-like transposases and interactions with Stowaway MITEs.
DNA-binding specificity of rice mariner-like transposases and interactions with Stowaway MITEs.
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水稻水晶样转座酶的DNA结合特异性以及与偷偷摸摸的螨虫的相互作用。
DOI：
10.1093/nar/gki509
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发表时间：
2005
影响因子：
14.9
通讯作者：
Wessler SR
中科院分区：
文献类型：
--
作者：
Feschotte C;Osterlund MT;Peeler R;Wessler SR
水手类元件(MLE)是在动植物王国中发现的DNA转座子。先前对水稻基因组序列的计算调查显示，34个全长MLEs属于25个不同的家族。这项调查还确定了Osmar分子和Stowaway螨超家族之间的序列相似性，导致了一个假说的提出，即偷渡者是由Osmar转座酶动员的。在这里，我们研究了两个OSMAR转座酶OSMAR5和OSMAR10的DNA结合活性和特异性。与其他水手类转座酶一样，OSMAR与其编码转座子的末端反向重复序列(TIR)特异结合。OSMAR5通过包含两个功能上可分离的螺旋-旋转-螺旋基序的两个N-末端结构域与DNA结合，类似于后生动物中Tc1样转座酶和PAX转录因子的配对结构域。此外，OSMAR的结合不仅限于它们自己的TIR；OSMAR5转座酶还可以在体外与来自紧密相关的OSMAR元件的TIR以及从水稻基因组序列中挖掘的几个Stowaway家族的TIR相互作用。这些结果首次为Osmar元件和Stowaway螨类之间的功能关系提供了生化证据，并使我们认为在单个真核生物基因组中共存的相关但不同的转座子家族之间存在广泛的串扰。
Mariner-like elements (MLEs) are DNA transposons found throughout the plant and animal kingdoms. A previous computational survey of the rice (Oryza sativa) genome sequence revealed 34 full length MLEs (Osmars) belonging to 25 distinct families. This survey, which also identified sequence similarities between the Osmar elements and the Stowaway superfamily of MITEs, led to the formulation of a hypothesis whereby Stowaways are mobilized by OSMAR transposases. Here we investigate the DNA-binding activities and specificities of two OSMAR transposases, OSMAR5 and OSMAR10. Like other mariner-like transposases, the OSMARs bind specifically to the terminal inverted repeat (TIR) sequences of their encoding transposons. OSMAR5 binds DNA through a bipartite N-terminal domain containing two functionally separable helix-turn-helix motifs, resembling the paired domain of Tc1-like transposases and PAX transcription factors in metazoans. Furthermore, binding of the OSMARs is not limited to their own TIRs; OSMAR5 transposase can also interact in vitro with TIRs from closely related Osmar elements and with consensus TIRs of several Stowaway families mined from the rice genome sequence. These results provide the first biochemical evidence for a functional relationship between Osmar elements and Stowaway MITEs and lead us to suggest that there is extensive cross-talk among related but distinct transposon families co-existing in a single eukaryote genome.
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影响因子：
1.5
作者：
Kapitonov, VV;Jurka, J
通讯作者：
Jurka, J
影响因子：
11.4
作者：
Lampe, DJ;Churchill, MEA;Robertson, HM
通讯作者：
Robertson, HM
DOI：
10.1073/pnas.91.11.4746
发表时间：
1994-05-24
影响因子：
11.1
作者：
FRANZ, G;LOUKERIS, TG;SAVAKIS, C
通讯作者：
SAVAKIS, C
影响因子：
3.5
作者：
Mazzone, M;De Gregorio, E;Di Nocera, PP
通讯作者：
Di Nocera, PP
影响因子：
11.6
作者：
BUREAU, TE;WESSLER, SR
通讯作者：
WESSLER, SR