Sequence polymorphism of HLA-DP beta chains.

Sequence polymorphism of HLA-DP beta chains.
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HLA-DP β 链的序列多态性。

DOI:
10.1007/bf00352845
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发表时间:
1989
期刊:
影响因子:
3.2
通讯作者:
Yunis,I
Yunis,I
中科院分区:
医学4区
文献类型:
--
作者:
Lee,JS;Sartoris,S;Briata,P;Choi,E;Cullen,C;Lepaslier,D;Yunis,I

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主要组织相容性复合体I类的N端结构域的多态性残基以及在较小程度上影响分子与肽抗原和t细胞受体的相互作用,并且是HLA限制效应靶细胞相互作用的基础。虽然已经对几种HLA-DRB1、DRB4、DQA1和dqb1等位基因的第一结构域进行了测序和分析,但对HLA-DPA1和DPB1基因的序列多态性知之甚少。此外,通过细胞检测,DP位点的多态性远低于DR和DQ。我们对HLA纯合子细胞系的HLAⅱ类cdna进行了DNA序列分析,以更精确地确定等位基因群内的多态性。从四种细胞系中获得编码DPB1链的全长cDNA克隆,并为第十届国际HLA研讨会进行分析。
Polymorphic residues in the N terminal domains of major histocompatibility complex class I and to a lesser extent classII c~ chains influence interactions of the molecules with peptide antigens and T-cell receptors, and are the basis for HLA restriction of effector to target cell interactions. While first domains of several HLA-DRB1, DRB4, DQA1, andDQB1 alleleshavebeen sequenced and analyzed, much less is known about sequence polymorphism of the HLA-DPA1 and DPB1 genes. In addition, polymorphismdetectedat the DP locus by cellular assays is much lower than that for DR and DQ. We undertook DNA sequences analysis of HLA class II cDNAs from HLA homozygous cell lines to define the polymorphisms within allelic groups more precisely. Full-length cDNA clones encoding DPB1 chains were obtained from four cell lines that were analyzed for the Tenth International HLA Workshop.
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