In vitro evaluation of a bone morphogenetic protein‑2 nanometer hydroxyapatite collagen scaffold for bone regeneration.
In vitro evaluation of a bone morphogenetic protein‑2 nanometer hydroxyapatite collagen scaffold for bone regeneration.
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DOI：
10.3892/mmr.2018.8579
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发表时间：
2018-04
影响因子：
3.4
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Cai Y;Tong S;Zhang R;Zhu T;Wang X
骨组织工程支架的制备和生物相容性是骨组织工程成功的关键。纳米羟基磷灰石（nHAP）与胶原（COL）的组合经常被用作合适的骨支架材料。此外，生长因子，包括骨形态发生蛋白-2（BMP-2），用于增强支架性能。本研究采用共混法和冷冻干燥法制备BMP-2-nHAP-COL支架。进行ELISA以确定BMP-2从支架的释放速率。流式细胞术用于鉴定大鼠骨髓间充质干细胞（BMSCs）之前，他们与支架组合。扫描电镜观察支架结构和BMSC种植后的形态。BMSCs也用于评估支架的体外生物相容性。与适当的对照组一起评估BMP-2-nHAP-COL和nHAP-COL支架。计数细胞以确定早期细胞粘附。细胞计数试剂盒-8和碱性磷酸酶法分别检测细胞增殖和分化。大体形态学证实BMP-2-nHAP-COL支架的微观结构符合骨组织工程支架的最佳特征。此外，BMP-2-nHAP-COL支架没有表现出生物毒性，并被证明可以促进BMSC粘附、增殖和分化。BMP-2-nHAP-COL支架在体外具有良好的生物相容性，因此可以进一步修饰以构建未来骨组织工程的优化支架。
Scaffold fabrication and biocompatibility are crucial for successful bone tissue engineering. Nanometer hydroxyapatite (nHAP) combined with collagen (COL) is frequently utilized as a suitable osseous scaffold material. Furthermore, growth factors, including bone morphogenetic protein-2 (BMP-2), are used to enhance the scaffold properties. The present study used blending and freeze-drying methods to develop a BMP-2-nHAP-COL scaffold. An ELISA was performed to determine the BMP-2 release rate from the scaffold. Flow cytometry was used to identify rat bone marrow-derived mesenchymal stem cells (BMSCs) prior to their combination with the scaffold. Scanning electron microscopy was used to observe the scaffold structure and BMSC morphology following seeding onto the scaffold. BMSCs were also used to assess the biological compatibility of the scaffold in vitro. BMP-2-nHAP-COL and nHAP-COL scaffolds were assessed alongside the appropriate control groups. Cells were counted to determine early cell adhesion. Cell Counting kit-8 and alkaline phosphatase assays were used to detect cell proliferation and differentiation, respectively. Gross morphology confirmed that the BMP-2-nHAP-COL scaffold microstructure conformed to the optimal characteristics of a bone tissue engineering scaffold. Furthermore, the BMP-2-nHAP-COL scaffold exhibited no biological toxicity and was demonstrated to promote BMSC adhesion, proliferation and differentiation. The BMP-2-nHAP-COL scaffold had good biocompatibility in vitro, and may therefore be modified further to construct an optimized scaffold for future bone tissue engineering.
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