Hot Spot-Localized Artificial Antibodies for Label-Free Plasmonic Biosensing.
Hot Spot-Localized Artificial Antibodies for Label-Free Plasmonic Biosensing.
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DOI：
10.1002/adfm.201202370
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发表时间：
2013-04-12
影响因子：
19
通讯作者：
Singamaneni, Srikanth
中科院分区：
文献类型：
--
作者：
Abbas, Abdennour;Tian, Limei;Morrissey, Jeremiah J.;Kharasch, Evan D.;Singamaneni, Srikanth
关键词：
近十年来，生物分子印迹技术的发展为用人工抗体替代天然抗体提供了广阔的前景。大量的报告致力于有机和无机纳米结构的印迹，但很少有对具有转导功能的纳米材料进行印迹的报道。本文描述了一种利用可逆模板固定化和硅氧烷共聚合的相对快速高效的等离子体热点局部化金纳米棒表面印迹技术。该技术能够在纳米尺度上对印迹过程进行精细控制，并提供具有高选择性和可重用性的纳米生物传感器。概念验证是通过使用局部表面等离子体共振光谱检测中性粒细胞明胶酶相关脂钙蛋白（NGAL）来建立的，NGAL是急性肾损伤的生物标志物。这项工作代表了等离子体纳米生物传感器与合成抗体无标记和成本效益的诊断分析的有价值的一步。我们期望这种新型表面印迹等离子体纳米材料将为推进等离子体纳米结构的生物医学应用开辟新的可能性。
The development of biomolecular imprinting over the last decade has raised promising perspectives in replacing natural antibodies with artificial antibodies. A significant number of reports have been dedicated to imprinting of organic and inorganic nanostructures, but very few were performed on nanomaterials with a transduction function. Herein we describe a relatively fast and efficient plasmonic hot spot-localized surface imprinting of gold nanorods using reversible template immobilization and siloxane co-polymerization. The technique enables a fine control of the imprinting process at the nanometer scale and provides a nanobiosensor with high selectivity and reusability. Proof of concept is established by the detection of neutrophil gelatinase-associated lipocalin (NGAL), a biomarker for acute kidney injury, using localized surface plasmon resonance spectroscopy. The work represents a valuable step towards plasmonic nanobiosensors with synthetic antibodies for label-free and cost-efficient diagnostic assays. We expect that this novel class of surface imprinted plasmonic nanomaterials will open up new possibilities in advancing biomedical applications of plasmonic nanostructures.
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