Active pathways of anaerobic methane oxidation across contrasting riverbeds.
Active pathways of anaerobic methane oxidation across contrasting riverbeds.
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对比河床厌氧甲烷氧化的活跃途径
DOI：
10.1038/s41396-018-0302-y
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Trimmer M
中科院分区：
文献类型：
--
作者：
Shen LD;Ouyang L;Zhu Y;Trimmer M
甲烷厌氧氧化（AOM）可以减少海洋生态系统中甲烷的排放，但河流中的AOM却鲜为人知，其在全球碳循环中的作用日益受到重视。我们测量了不同的电子受体，包括亚硝酸盐，硝酸盐，硫酸盐和三价铁驱动的AOM电位，并确定了不同河床的微生物。AOM活动仅限于减少，桑迪河床，而没有活动的减少，砾石河床，那里有很少的厌氧甲烷氧化菌测量。所有桑迪河床中均发生了亚硝酸盐依赖的AOM和硝酸盐依赖的AOM，最大速率分别为61.0和20.0 nmol CO2 g −1（干沉积物）d−1，而硫酸盐依赖的AOM和三价铁依赖的AOM仅发生在甲烷浓度最高且AOM途径多样性最大的地方。Methylomirabilis oxyfera（M. oxyfera）类细菌和甲烷产气菌Methanoperedens nitroreducens（M.在桑迪河床中检测到了类似硝化还原菌的古菌（16 S rRNA基因丰度分别为9.3 × 105 ~ 1.5 × 107和2.1 × 104 ~ 2.5 × 105拷贝/g-1干沉积物），但没有发现其他已知的厌氧甲烷氧化菌。然后，我们找到了M。oxyfera-like细菌和M. nitroreducens样古菌积极参与亚硝酸盐和硝酸盐/三价铁依赖AOM。因此，我们证明了多种途径的AOM甲烷，虽然活动ofM。oxyfera-like细菌和M.硝基还原菌类古菌占优势。
Anaerobic oxidation of methane (AOM) reduces methane emissions from marine ecosystems but we know little about AOM in rivers, whose role in the global carbon cycle is increasingly recognized. We measured AOM potentials driven by different electron acceptors, including nitrite, nitrate, sulfate, and ferric iron, and identified microorganisms involved across contrasting riverbeds. AOM activity was confined to the more reduced, sandy riverbeds, whereas no activity was measured in the less reduced, gravel riverbeds where there were few anaerobic methanotrophs. Nitrite-dependent and nitrate-dependent AOM occurred in all sandy riverbeds, with the maximum rates of 61.0 and 20.0 nmol CO2g−1(dry sediment) d−1, respectively, while sulfate-dependent and ferric iron-dependent AOM occurred only where methane concentration was highest and the diversity of AOM pathways greatest. DiverseCandidatusMethylomirabilis oxyfera (M. oxyfera)-like bacteria andCandidatusMethanoperedens nitroreducens (M. nitroreducens)-like archaea were detected in the sandy riverbeds (16S rRNA gene abundance of 9.3 × 105to 1.5 × 107and 2.1 × 104to 2.5 × 105copies g−1dry sediment, respectively) but no other known anaerobic methanotrophs. Further, we foundM. oxyfera-like bacteria andM. nitroreducens-like archaea to be actively involved in nitrite- and nitrate/ferric iron-dependent AOM, respectively. Hence, we demonstrate multiple pathways of AOM in relation to methane, though the activities ofM. oxyfera-like bacteria andM. nitroreducens-like archaea are dominant.
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影响因子：
3.3
作者：
Hu, Shihu;Zeng, Raymond J.;Yuan, Zhiguo
通讯作者：
Yuan, Zhiguo
影响因子：
4.6
作者：
Kojima H;Tokizawa R;Kogure K;Kobayashi Y;Itoh M;Shiah FK;Okuda N;Fukui M
通讯作者：
Fukui M
影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
DOI：
10.1073/pnas.0902037106
发表时间：
2009-05-19
影响因子：
11.1
作者：
Canfield, Donald E.;Farquhar, James
通讯作者：
Farquhar, James
DOI：
10.1073/pnas.1411617111
发表时间：
2014-12-23
影响因子：
11.1
作者：
Deutzmann, Joerg S.;Stief, Peter;Schink, Bernhard
通讯作者：
Schink, Bernhard